自制鼻咽解毒胶囊含药血清对鼻咽癌细胞系5-8F存活、凋亡的影响及其机制  

Effects and mechanism of self-made Biyan Jiedu capsule-medicated serum on survival and apoptosis of nasopharyngeal cancer cell line 5-8F

作　　者：韩蜜[1,2] 龙远雄 HAN Mi;LONG Yuanxiong(Department of Pharmacy,Hunan Cancer Hospital,Changsha 410013,China;不详)

机构地区：[1]湖南省肿瘤医院药学部,长沙410013 [2]湖南中医药大学第一附属医院科研部

出　　处：《山东医药》2023年第36期31-36,共6页Shandong Medical Journal

基　　金：湖南省自然科学基金项目(2022JJ70104);湖南省教育厅科学研究优秀青年项目(21B0372);湖南省卫健委科研计划项目一般资助课题(D202302048885)。

摘　　要：目的观察自制鼻咽解毒胶囊含药血清对鼻咽癌细胞系5-8F存活、凋亡的影响,并探讨其机制。方法将对数生长期5-8F细胞分为8组,5%、10%、15%、20%、25%、30%鼻咽解毒胶囊含药血清组分别加入5%、10%、15%、20%、25%、30%鼻咽解毒胶囊含药血清,20%空白血清组加入20%不含药血清,对照组细胞正常培养,培养24、48 h,CCK-8法检测各组细胞存活率。将对数生长期5-8F细胞分为4组,低剂量组加入5%鼻咽解毒胶囊含药血清,中剂量组加入20%鼻咽解毒胶囊含药血清,高剂量组加入30%鼻咽解毒胶囊含药血清,阴性组细胞正常培养,培养24 h,流式细胞术检测细胞凋亡率,RT-qPCR法检测细胞中K-RAS、RAF mRNA相对表达量,Western blot法检测细胞中K-RAS、RAF、p-ERK1/2蛋白相对表达量。结果与对照组比较,20%含药血清组、25%含药血清组、30%含药血清组培养24 h及48 h的细胞存活率降低(P均<0.05);与20%空白血清组比较,25%含药血清组培养48 h和30%含药血清组培养24、48 h的细胞存活率降低(P均<0.05);与同组24 h比较,30%含药血清组培养48 h的细胞存活率降低(P<0.05)。与阴性组比较,低剂量组、中剂量组、高剂量组凋亡率升高(P均<0.05);与低剂量组比较,中剂量组、高剂量组细胞凋亡率升高(P均<0.05);与中剂量组比较,高剂量组细胞凋亡率升高(P<0.05)。与阴性组比较,低剂量组、中剂量组、高剂量组的K-RAS、RAF mRNA相对表达量降低(P均<0.05);与低剂量组比较,中剂量组、高剂量组K-RAS、RAF mRNA相对表达量降低(P均<0.05);与中剂量组比较,高剂量组K-RAS、RAF mRNA相对表达量降低(P均<0.05)。与阴性组比较,高剂量组K-RAS蛋白、RAF蛋白、p-ERK1/2蛋白相对表达量降低,中剂量组的K-RAS、RAF蛋白相对表达量降低,低剂量组RAF蛋白相对表达量降低(P均<0.05);与低剂量组比较,高剂量组K-RAS、RAF、p-ERK1/2蛋白相对表达量及中剂量组K-RAS、p-ERK1/2蛋白相对表达量降�Objective To observe the effects of self-made Biyan Jiedu capsule-medicated serum on the survival and apoptosis of nasopharyngeal carcinoma cell line 5-8F,and to explore its mechanism.Methods The 5-8F cells in the logarithmic growth phase were divided into eight groups;cells in the 5%,10%,15%,20%,25%and 30%Biyan Jiedu capsule-medicated serum groups were added with 5%,10%,15%,20%,25%and 30%Biyan Jiedu capsules,cells in the 20%blank serum group were gavaged with 20%drug-free serum,cells in the control group were cultured normally;they were cultured for 24 and 48 h,and the cell viability of each group was detected by CCK-8.The 5-8F cells in the logarithmic growth phase were divided into four groups;cells in the low-dose group were supplemented with 5%Biyan Jiedu capsule-medicated serum,the medium-dose group with 20%Biyan Jiedu capsule-medicated serum,the high-dose group with 30%Biyan Jiedu capsule-medicated serum,while cells in the control group were cultured normally for 24 h,and the apop-tosis rate was detected by flow cytometry.RT-qPCR was used to detect the relative expression levels of K-RAS and RAF mRNA in cells.Western blotting was used to detect the relative expression levels of K-RAS,RAF and p-ERK1/2 proteins in cells.Results Compared with the control group,the cell survival rates of the 20%,25%,and 30%Biyan Jiedu capsule-medicated serum groups decreased after 24 and 48-hour culture(all P<0.05).Compared with the 20%blank serum group,the cell survival rates decreased in the 25%Biyan Jiedu capsule-medicated serum group after 48-hour culture and the 30%Biyan Jiedu capsule-medicated serum group after 24-and 48-hour culture(all P<0.05).Compared with the same group at 24 h,the cell survival rate of the 30%Biyan Jiedu capsule-medicated serum group was reduced at 48 h(P<0.05).Compared with the negative group,the apoptosis rates of the low-dose group,the medium-dose group and the high-dose group increased(all P<0.05);compared with the low-dose group,the apoptosis rates of the medium-dose group and the high-dose grou

关 键 词：鼻咽解毒胶囊 鼻咽癌 5-8F细胞 细胞凋亡 MAPK/ERK信号通路 

分 类 号：R965.2[医药卫生—药理学]