绵羊iNOS基因突变与布鲁杆菌侵染率的关系分析  

Relationship Between iNOS Gene Polymorphism and Brucella Susceptibility in Kazakh Sheep

作　　者：何营 徐杰 周永顺 韩凯 李刚[1] 陈福龙[1] 高剑峰[1] HE Ying;XU Jie;ZHOU Yongshun;HAN Kai;LI Gang;CHEN Fulong;GAO Jianfeng(College of Life Sciences,Shihezi University,Shihezi 832003,China)

机构地区：[1]石河子大学生命科学学院,石河子832003

出　　处：《中国动物传染病学报》2023年第5期14-22,共9页Chinese Journal of Animal Infectious Diseases

基　　金：国家重点基础研究发展计划(973计划)项目(2010CB530200)。

摘　　要：探究绵羊诱导型一氧化氮合酶(iNOS)基因多态性与布鲁菌易感性的关系。使用虎红平板血清凝集试验检测哈萨克羊布鲁菌患病情况。参考GenBank中绵羊iNOS基因序列,针对其15、16、17外显子及其邻近内含子片段设计引物,PCR扩增出目的基因片段。采用SSCP凝胶电泳检测不同基因型分型,DNA测序进行多态性检测,分析其SNPs与哈萨克羊布鲁菌易感性之间的关系。经检测发现阳性羊数67只,占比29.00%。在哈萨克羊iNOS基因的exon 15片段上有F15-C29002T和F15-G29076A两个多态位点。在exon 16片段上有F16-A30045G位点。在exon 17片段上有F17-T31213G位点。卡方检验表明,F15-C29002T和F15-G29076A多态位点与布鲁菌易感性的相关性不显著(P>0.05),F16-A30045G和F17-T31213G多态位点与布鲁菌易感性的相关性极显著(P<0.01)。哈萨克羊iNOS基因F15-C29002T和F15-G29076A与布鲁菌易感性没有相关性,F16-A30045G和F17-T31213G与布鲁菌易感性存在相关性。To investigate the relationship between iNOS gene polymorphism and Brucella susceptibility in Kazakh sheep,primers were designed for exons 15,16,17 and their adjacent introns according to the iNOS gene sequences in GenBank and the target gene fragments were amplifi ed by PCR.SSCP gel electrophoresis was used to detect different genotypes and DNA sequencing was used to detect polymorphism for analysis of relationship between SNPs and Brucella Kazakh susceptibility.Total 67 sheep were found positive,accounting for 29.00%.Two polymorphic loci F15-C29002T and F15-G29076a were found in exon 15 fragment,f16-A30045G locus in exon 16 fragment and f17-T31213G locus on exon 17 fragment of iNOS gene of Kazakh sheep.Chi-square test showed that polymorphic loci f15-C29002T and F15-G29076A had no signifi cant correlation with Brucella susceptibility(P>0.05)while F16-A30045g and F17-T31213g had very signifi cant correlation with Brucella susceptibility(P<0.01).In conclusion,polymorphic loci F15-C29002T and F15-G29076A of iNOS gene of Kazakh sheep had no correlation with Brucella susceptibility while F16-A30045G and F17-T31213G had correlation with Brucella susceptibility.

关 键 词：哈萨克羊 INOS基因 PCR-SSCP SNPs 布鲁菌 

分 类 号：S858.26[农业科学—临床兽医学]