肌幼虫期旋毛虫感染小鼠肠道菌群的变化分析  

Changes of Intestinal Microflora in Mice Infected with Trichinella Spiralis at Muscle Larval Stage

作　　者：康杰[1] 仝明薇 杨勇 KANG Jie;TONG Mingwei;YANG Yong(Science and Technology Center of Fenyang College,Shanxi Medical University,Fenyang 032200,China;School of Basic Medical Science,Shan Xi Medical University,Taiyuan 030000,China)

机构地区：[1]山西医科大学汾阳学院科技中心,汾阳032200 [2]山西医科大学基础医学院,太原030000

出　　处：《中国动物传染病学报》2023年第5期23-30,共8页Chinese Journal of Animal Infectious Diseases

基　　金：山西医科大学汾阳学院科技扶持基金项目(2020C03);山西省基础研究计划资助项目(20210302123297)。

摘　　要：本研究旨在分析肌幼虫期旋毛虫感染小鼠肠道菌群的结构组成变化。选取12只雌性BALB/c小鼠,随机分为对照组(CK)和感染组(Ts35),感染组小鼠经口感染300条旋毛虫肌幼虫,对照组口服等量PBS。收集对照组和感染组小鼠在感染后第35 d的盲肠内容物,提取DNA,并对其进行16S rRNA测序及生物信息学分析。结果发现,对照组小鼠和感染组小鼠肠道菌群的Alpha多样性没有显著差异,而β多样性发生变化,表明两组的菌群结构不同。Metastats分析发现,与CK组相比,旋毛虫感染35 d后的小鼠肠道中产丁酸的罗斯拜瑞氏菌(Roseburia)和抗炎及组织修复相关的梭菌属(Clostridium)丰度显著升高(P<0.05),而拟杆菌(Bacteroides)(P<0.01)、乳酸杆菌(Lactobacillus)和Ralstonia(P<0.05)的丰度显著下降。同样地,LEfSe分析发现罗斯拜瑞氏菌的丰度明显升高(P<0.05)。综上表明,肌幼虫期旋毛虫感染小鼠肠道菌群的组成与对照组有明显的不同。In this study,the changes of the gut microbiota in mice were investigated by infection with Trichinellaspiralis at the muscle larvae stage.Twelve SPF female BALB/c mice were randomly divided into control group and infection group.BALB/c mice in the infection group were orally inoculated with 300T.spiralismuscle larvae and those in control group were given PBS.Mice were sacrificed at 35 day post-infection and their cecal contents were collected for DNA extraction.The composition of gut microbiota was analyzed by sequencing of 16S rRNA and bioinformatics methods.The results showed that there was no significant difference in the alpha diversity of the intestinal microbiota between the control and infected micewhile the beta diversity was changed,indicating that the two groups had different microbiota structures.Metastats analysis showed that the abundance of Roseburiathat produced butyrate and Clostridium that related with anti-inflammatory and tissue repair was significantly(P<0.05)increased in intestines of mice infected with T.spiralis,compared with control group.However,the abundance of Bacteroides(P<0.01),Lactobacillus and Ralstonia(P<0.05)were significantly decreased.Moreover,LEfSe analysis showed that the abundance of Roseburia was significantly increased(P<0.05).In conclusion,the composition of gut microbiota in mice infected with T.spiralis during muscle larva stage was significantly different from that of normal mice.

关 键 词：旋毛虫 肌幼虫 肠道菌群 宿主寄生虫相互作用 

分 类 号：S855.9[农业科学—临床兽医学]