广西猪繁殖与呼吸综合征病毒感染情况调查及其欧洲型毒株ORF5基因变异分析  

Investigation on the Infection Status of Porcine Reproductive and Respiratory Syndrome Virus and Mutation Analysis on ORF5 Gene of its European Type Virus

作　　者：赵硕 段群棚 何颖 卢冰霞 许心婷 秦毅斌 李斌 全琛宇 梁家幸 周英宁 蒋冬福 陈婷婷 卢敬专 陈忠伟 赵武 ZHAO Shuo;DUAN Qunpeng;HE Ying;LU Bingxia;XU Xinting;QIN Yibin;LI Bin;QUAN Chenyu;LIANG Jiaxing;ZHOU Yingning;JIANG Dongfu;CHEN Tingting;LU Jingzhuan;CHEN Zhongwei;ZHAO Wu(Key Laboratory of China(Guangxi)-ASEAN Cross-border Animal Disease Prevention and Control,Ministry of Agriculture and Rural Affairs,key Laboratory of Veterinary Biotechnology of Guangxi,Guangxi Veterinary Research Institute,Nanning 530001,China)

机构地区：[1]广西壮族自治区兽医研究所广西兽医生物技术重点实验室农业农村部中国(广西)-东盟跨境动物疫病防控重点实验室,南宁530001

出　　处：《中国动物传染病学报》2023年第5期135-144,共10页Chinese Journal of Animal Infectious Diseases

基　　金：广西创新驱动发展专项资金项目(桂科AA17204057,桂科AA18118051);广西基本科研业务费专项(桂科专项21-3);柳州市科技计划项目(2020NACB0804);南宁市科学研究与技术开发计划项目(20202090);南宁市西乡塘区科学研究与技术开发计划项目(2020021605)。

摘　　要：为了解广西近期猪繁殖与呼吸综合征病毒(PRRSV)感染情况及其欧洲型PRRSVORF5的遗传变异特点,2020年6月至2021年5月,采集广西14个设区市疑似猪繁殖与呼吸综合征(PRRS)发病猪病料、血清共782份,进行PRRSV的qRT-PCR分型检测及ORF5基因扩增,并运用SPSS23.0、DNAStar、MEGA X等生物学软件对检测结果及获得的PRRSVORF5基因序列进行分析。结果显示:样品检测总阳性率为29.54%,其中欧洲型(PRRSV-1)PRRSV阳性率为6.78%,美洲型(PRRSV-2)PRRSV阳性率为22.76%,表明受检猪群普遍存在PRRSV感染;在不同设区市中,贵港、崇左感染率最高(50.00%);在不同季度中,冬季感染率最高(41.00%);在不同年龄阶段猪群中,保育猪感染率最高(53.42%);感染主要以PRRSV-2为主,新发PRRSV-1感染;广西新发PRRSV-1在ORF5基因上存在相同独特的核苷酸及氨基酸变异特点,与中国香港分离欧洲株HKEU16亲缘关系较近,可能是在引种等贸易过程中所引进,并在饲养环境、免疫压力等多种因素的作用下发生了一定的变异;广西近期受检猪群普遍存在PRRSV-2感染,新出现PRRSV-1的感染,猪场应重视并及时调整、实施针对性的PRRS防控计划。To study the infection status of PRRSV and genetic variation on ORF5 gene of European type PRRSV in Guangxi,782 tissue and serum samples of suspected PRRS-infected pigs in 14 cities were collected and tested by qRT-PCR and genotyping.ORF5 gene of positive simples were amplified,sequenced and analyzed by DNAStar and MEGA X.The results showed that the positive rate of suspected simples was 29.54%,positive rate of European-type PRRSV(PRRSV-1)was 6.78%while North American-type PRRSV(PRRSV-2)was 22.76%.PRRSV infection of pigs was common in the tested herds,of which the highest infection rate(50.00%)was found in Guigang and Chongzuo.In different seasons,the highest infection rate(41.00%)occurred in winter.Among different pig herds,nursery pigs had the highest infection rate(53.42%).PRRSV-2 were dominant and PRRSV-1 was discovered recently.TheORF5gene of the newly discovered European-type PRRSV in Guangxi had the same unique nucleotide and amino acid variation characteristics.These strains had closer relationship with European strain HKEU16 isolated from Hong Kong.The new European-type PRRSV in Guangxi might have been introduced during the trade process and had undergone certain mutations under the influence of various factors such as breeding environment and immune pressure.In a recent investigation in Guangxi,the North American-type PRRSV infection was found widespread while European-type PRRSV infection just newly emerged.Therefore,pig farms should pay more attention to adjust and implement targeted PRRS prevention and control plans.

关 键 词：猪繁殖与呼吸综合征病毒 感染情况 欧洲型 ORF5基因 遗传变异分析 

分 类 号：S852.651[农业科学—基础兽医学]