四川九龙县牦牛源蜱感染SFGR分子流行病学调查  

Molecular Detection of SFGR in Ticks Collected from Yaks in Jiulong County of Sichuan Province,China

作　　者：肖晨冬 袁东波[2] 尹念春 张枥元 陈亮 郝力力[1] XIAO Chendong;YUAN Dongbo;YIN Nianchun;ZHANG LIyuan;CHEN Liang;HAO Lili(College of Animal&Veterinary Sciences,Southwest Minzu University,Chengdu 610041,China;Center for Animal Disease Control and Prevention in Sichuan Province,Chengdu 61004l,China;Center for Animal Disease Control and Prevention in Suining,Suining 629000,China;Animal Health Supervision Institution in Deyang,Deyang 618000,China;Center for Animal Disease Control and Prevention in Deyang,Deyang 618000,China)

机构地区：[1]西南民族大学畜牧兽医学院,成都610041 [2]四川省动物疫病预防控制中心,成都610041 [3]遂宁市动物疫病预防控制中心,遂宁629000 [4]德阳市动物卫生监督所,德阳618000 [5]德阳市动物疫病预防控制中心,德阳618000

出　　处：《中国动物传染病学报》2023年第5期159-167,共9页Chinese Journal of Animal Infectious Diseases

基　　金：西南民族大学研究生创新型科研项目(CX2020SZ56)。

摘　　要：为了解四川省九龙县牦牛体表寄生蜱的种类及其斑点热群立克次体(SFGR)的感染情况。采集牦牛体表的蜱,经形态学初步鉴定后,提取蜱总DNA,PCR扩增蜱ITS-2基因及SFGRompA、ompB基因,并对扩得的阳性产物进行测序和构建进化树分析,从而确定蜱及其携带SFGR的种类。在九龙县3个乡镇共采集到蜱585只,其中微小扇头蜱占52.65%(308/585)、卵形硬蜱占32.99%(193/585)、锐跗硬蜱和西藏革蜱分别占8.89%(52/585)和5.50%(32/585)。所有蜱中有374只检出SFGR,总感染率为63.93%(374/585),其中半农牧区(70.60%)的感染率极显著的高于纯牧区(45.10%)(P<0.01)。本研究首次对九龙县牦牛体表寄生的蜱虫种类及SFGR流行情况进行了调查,蜱传SFGR感染率较高且感染的SFGR主要为饶氏立克次体。In order to investigate tick species and spotted fever grouprickettsia(SFGR)infection in ticks collected from yaks in Jiulong county of Sichuan province,ticks were collected from yaks and classified by morphological identification.Total DNA of ticks was extracted and partial sequences of ITS-2 of ticks,ompA and ompB genes of SFGR were amplified by PCR,respectively.The positive products were sequenced and compared through the NCBI database and phylogenetic trees were constructed based on ITS-2,ompA and ompBfor determination of tick and SFGR species,respectively.The results showed that a total of 585 ticks were collected and the most abundant species was R.microplus(52.65%,308/585),followed by I.ovatus(32.99%,193/585),I.acutitarsus(8.89%,52/585)and D.everestianus(5.50%,32/585).In ticks collected from yaks,SFGR DNA was detected with a positive rate of 63.93%(374/585).The infection rate in semi-agricultural and pastoral areas(70.60%)was significantly(P<0.01)higher than that in pastoral areas(45.10%).This is thefirst report on the prevalence of SFGR in Jiulong county,which suggested a high infection rate of SFGR in ticks,mainly in R.raoultii.

关 键 词：斑点热群立克次体 牦牛 蜱 分子流行病学 九龙县 

分 类 号：S858.23[农业科学—临床兽医学]