牦牛源蜡样芽胞杆菌的分离鉴定及耐药性分析  

Isolation,Identifi cation and Drug Resistance of Bacillus Cereus from Yaks

作　　者：王俊书 朱杰[2] 郭宏元 徐进强 杨晶晶[1] 金红岩[1] WANG Junshu;ZHU Jie;GUO Hongyuan;XU Jinqiang;YANG Jingjing;JIN Hongyan(Key Laboratory of Veterinary Medicine,College of Animal Science and Technology,Tibet Vocational and Technical College,Lhasa 850030,China;Shanghai Institute of Veterinary Medicine,CAAS,Shanghai 200241,China)

机构地区：[1]西藏职业技术学院动物科学技术学院兽医重点实验室,拉萨850030 [2]中国农业科学院上海兽医研究所,上海200241

出　　处：《中国动物传染病学报》2023年第5期181-187,共7页Chinese Journal of Animal Infectious Diseases

基　　金：2017年西藏自治区科研创新团队项目;2019年西藏自治区科技厅项目(XZ2019ZRG-149)。

摘　　要：为了研究牦牛源蜡样芽胞杆菌致病性和耐药性,本实验对其进行了细菌形态学观察、16S rDNAPCR和序列比对、动物回归试验及药敏分析。结果显示:56份牦牛奶样,分离出135株细菌,对分离菌进行16S rDNAPCR并测序,其中有42株为蜡样芽孢杆菌,蜡样芽胞杆菌检出率为75%(42/56),占总检出细菌的31.11%(42/135);其中分离菌株B201916S rDNAPCR测序结果与GenBank中蜡样芽胞杆菌的同源性为88.6%~99.3%,运用Mega7.0将分离菌株B2019与6株参考菌株的16S rDNA序列进行同源性比对分析,并构建系统进化树;在动物回归试验中小鼠腹泻,剖检小鼠发现肝脏肿大,肺脏边缘有出血;药敏试验结果显示,分离菌株对麦迪霉素、克林霉素、红霉素、庆大霉素、卡那霉素、丁胺卡那霉素、新霉素、万古霉素、氧氟沙星、环丙沙星、呋喃唑酮和米诺环素等13种抗生素表现出高度敏感,对多粘菌素B表现为中介,对其他16种抗生素均表现为耐药。试验结果表明该分离菌株是一株具有较强致病性的蜡样芽胞杆菌。In order to study the prevalence and drug resistance ofBacilluscereusfrom yaks,this experiment was carried out for bacterial morphology observation,16S rDNA PCR and sequence comparison,animal regression test and drug susceptibility analysis.The results showed that 135 bacterial strains of were isolated from 56 yak milk samples.Then these isolated strains were tested using 16S rDNA PCR and sequenced.As a result,42 strains were identified asB.cereuswith the detection rate of 75%(42/56),Accounting for 31.11%(42/135)of the total isolated bacteria.In addition,the 16S rDNA homology between the isolated strain B2019 with B.cereus in GenBank was 88.6%-99.3%.The 16S rDNA sequence of the isolated strain B2019 and 6 reference strains were compared with Mega7.0 for phylogenetic analysis.In the animal regression test,the inoculated mice had diarrhea.The autopsy of the infected mice found enlarged livers and bleeding lungs on the edges.Drug susceptibility testing showed that the isolated strains were highly susceptible to 13 antibiotics including mideacin,clindamycin,erythromycin,gentamicin,kanamycin,amikacin,neomycin,vancomycin,ofloxacin,ciprofloxacin,furazolidone and minocycline,medium susceptible to polymyxin B and resistant to the other 16 antibiotics.The test results showed that the isolated strain belonged to strongly pathogenicB.cereus.

关 键 词：蜡样芽孢杆菌 分离鉴定 16SrDNA 耐药性 

分 类 号：S852.61[农业科学—基础兽医学]