2018—2021年青藏高原部分地区牛羊戊型肝炎病毒血清学检测与分析  

Serological Detection and Analysis of Hepatitis E Virus in Cattle and Sheep in Selected Areas of the Qinghai-Tibet Plateau from 2018 to 2021

作　　者：娄永志 卢姊豪 武琦 陈姝宇 常攀 格桑卓玛 索朗斯珠 贡嘎 LOU Yongzhi;LU Zihao;WU qi;CHEN Shuyu;CHANG Pan;GE Sangzhuoma;SUO Langsizhu;GONG Ga(Animal Science College,Tibet Agriculture and Animal Husbandry University,Linzhi 860000,China;Animal Epidemic Prevention and Control Center of Tibet Autonomous Region,Tibet 850000,China)

机构地区：[1]西藏农牧学院动物科学学院,林芝860000 [2]西藏自治区动物疫病预防控制中心(西藏自治区畜牧总站),拉萨850000

出　　处：《中国动物传染病学报》2023年第5期202-205,共4页Chinese Journal of Animal Infectious Diseases

基　　金：国家自然基金项目(32060792)。

摘　　要：为了解青藏高原部分地区反刍动物戊型肝炎病毒的流行情况,本文采用间接ELISA法对2018—2021年采集的共计360份牦牛及藏羊血清(西藏那曲牦牛、藏羊血清各120份,青海牦牛血清120份)进行HEV血清学检测,并使用SPSS 23.0软件对不同地区、不同动物检测结果进行统计学分析。结果显示,青海、那曲两地牦牛戊型肝炎IgG抗体总阳性率为14.58%,两地IgG阳性率分别为13.33%(青海)、15.83%(那曲),那曲藏羊IgG抗体总阳性率为5.00%。青海牦牛各年份IgG抗体阳性率为6.67%、6.67%、20.00%、20.00%,那曲牦牛各年份IgG抗体阳性率为23.33%、13.33%、10.00%、16.67%,那曲藏羊各年份IgG抗体阳性率为0.00%、6.67%、3.33%、10.00%。统计学分析结果表明:4年来那曲牦牛HEV-IgG抗体阳性率(15.83%)显著于青海牦牛(13.33%)P≤0.05,4年来那曲牦牛HEV-IgG抗体阳性率(15.83%)极显著于那曲藏羊(5.00%)P≤0.01,4年来那曲牦牛HEV-IgM抗体阳性率(5.00%)显著于那曲藏羊(2.50%)P≤0.05。本研究结果为了解青藏高原反刍动物戊型肝炎血清流行病学情况奠定了基础。To understand the prevalence of hepatitis E virus(HEV)in some areas of the Qinghai-Tibetan Plateau,serum samples were collected from 2018 to 2021 from120 Tibetan yaks,120 Tibetan goats and 120 Qinghai yaks and tested for HEV antibodies using indirect ELISA method.As a result,total HEV IgG positive rate of yaks was 14.58%,of which the positive IgG rate was 13.33%in Qinghai,15.83%in Naqu.The positive IgG rate was 5.00%for Tibetan sheep in Naqu.Moreover,the positive IgG rates from 2018 to 2021 were 6.67%,6.67%,20.00%and 20.00%for yaks in Qinghai,23.33%,13.33%,10.00%and 16.67%for yaks in Naqu,and0.00%,6.67%,3.33%,10.00%for Tibetan sheep in Naqu.Statistical analysis showed that the positive rate of HEV IgG antibodies of yaks in Naqu(15.83%)was significantly higher than the Qinghai yak positive rate(13.33%)in the last 4 years.The positive rate of HEV IgG antibodies for yaks in Naqu(15.83%)was extremely significant(P≤0.01)than that of Tibetan sheep(5.00%)in the past 4 years.Additionally,the positive rate of HEV IgM antibodies in Naqu yaks(5.00%)was significantly(P≤0.05)higher than that of Tibetan sheep(2.50%)in the past 4 years,.These results laid a solid foundation for understanding the seroepidemiology of hepatitis E in ruminants on the Qinghai-Tibet Plateau.

关 键 词：青藏高原 反刍动物 戊型肝炎病毒 检测与分析 

分 类 号：S858.26[农业科学—临床兽医学]