金属离子对黑翅土白蚁消化代谢的影响  

Effects of metal ions on digestion and metabolism of Odontotermes formosanus

作　　者：漆梦雯 沈毅[1] 羊桂英 余婷[1] 李吴晗 周琪欢 谢晓俊 朱娅宁 莫建初[1] QI Mengwen;SHEN Yi;YANG Guiying;YU Ting;LI Wuhan;ZHOU Qihuan;XIE Xiaojun;ZHU Yaning;MO Jianchu(Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests,Institute of Insect Sciences,Zhejiang University,Hangzhou 310058,Zhejiang,China)

机构地区：[1]浙江大学昆虫科学研究所农业部作物病虫害分子生物学重点实验室,浙江杭州310058

出　　处：《浙江农林大学学报》2024年第1期154-160,共7页Journal of Zhejiang A&F University

基　　金：国家自然科学基金资助项目(31770686)。

摘　　要：【目的】探究金属离子对黑翅土白蚁Odontotermes formosanus消化代谢过程的影响,挖掘黑翅土白蚁对含有金属离子的厨余垃圾和农林废弃物进行资源化处理的潜在价值。【方法】根据浓度梯度法,将含有不同质量分数Al^(3+)、Ca^(2+)、Fe^(3+)和Mg^(2+)的饵料供给黑翅土白蚁,确定黑翅土白蚁对饵料中金属离子的最大可取食质量分数。采用电感耦合等离子体发射光谱仪(ICP-OES)测定黑翅土白蚁虫体内及其新建菌圃内的对应金属元素质量分数,明确饵料中Al^(3+)、Ca^(2+)、Fe^(3+)和Mg^(2+)对虫体内及新建菌圃内对应金属元素质量分数的影响。采用试剂盒法测定黑翅土白蚁虫体内及其新建菌圃内漆酶和纤维素酶的活性,确定饵料中Al^(3+)、Ca^(2+)、Fe^(3+)和Mg^(2+)对上述酶活性的影响。【结果】黑翅土白蚁对饵料中Al^(3+)、Ca^(2+)和Fe^(3+)的最大可取食质量分数均为1.00 g·kg^(-1),对饵料中Mg^(2+)的最大可取食质量分数为10.00 g·kg^(-1)。黑翅土白蚁取食含有10.00 g·kg^(-1) Mg^(2+)的饵料后,其虫体内和菌圃内都发生了Mg的富集;取食含有1.00 g·kg^(-1) Fe^(3+)的饵料后,Fe仅在菌圃内富集。取食含有1.00 g·kg^(-1) Al^(3+)的饵料,会显著提高黑翅土白蚁虫体内的漆酶活性(P<0.05);取食含有1.00 g·kg^(-1) Ca^(2+)的饵料,会显著提高黑翅土白蚁虫体内的漆酶活性(P<0.05),但会显著降低纤维素酶活性(P<0.05);取食含有1.00 g·kg^(-1) Fe^(3+)的饵料,会显著提高黑翅土白蚁虫体内的漆酶活性和菌圃中的纤维素酶活性(P<0.05),但会显著降低菌圃中的漆酶活性和黑翅土白蚁虫体内的纤维素酶活性(P<0.05);取食含有10.00 g·kg^(-1) Mg^(2+)的饵料,会显著提高黑翅土白蚁虫体内的纤维素酶活性和菌圃中的漆酶活性(P<0.05),但会显著降低菌圃中的纤维素酶活性(P<0.05)。【结论】黑翅土白蚁可取食分别含有1.00 g·kg^(-1) Al^(3+)、Ca^(2+)和Fe^(3+)的饵料和含[Objective]This study aims to explore the role of metal ions in influencing the digestive generation process of Odontotermes formosanus,which is beneficial to explore the potential value of resource treatment of agricultural and forestry waste and kitchen waste containing metal ions by O.formosanus.[Method]According to the concentration gradient method,the bait containing different mass fraction of Al^(3+),Ca^(2+),Fe^(3+)and Mg^(2+)was fed to O.formosanus to determine the maximum edible mass fraction of metal ions in bait for O.formosanus.ICP-OES was used to measure the corresponding metal elements in O.formosanus body and new-built fungus combs,and to obtain the effects of feeding baits containing Al^(3+),Ca^(2+),Fe^(3+)and Mg^(2+)on the corresponding metal elements mass fraction in O.formosanus body and new-built fungus combs.The activities of laccase and cellulase in O.formosanus body and new-built fungus combs were determined by kit method,and the effects of feeding baits containing Al^(3+),Ca^(2+),Fe^(3+)and Mg^(2+)on the activities of these enzymes were determined.[Result]The maximum edible mass fraction of Al^(3+),Ca^(2+)and Fe^(3+)in baits of O.formosanus was 1.00 g·kg^(-1),and Mg^(2+)in baits was 10.00 g·kg^(-1).Mg enrichment occurred both in O.formosanus body and new-built fungus combs after feeding baits containing 10.00 g·kg^(-1) Mg^(2+);Fe was only enriched in new-built fungus combs after feeding baits containing 1.00 g·kg^(-1) Fe^(3+).The laccase activity in O.formosanus body improved significantly after feeding baits containing 1.00 g·kg^(-1) Al^(3+).The laccase activity in O.formosanus body improved significantly,but the cellulase activity reduced significantly after feeding baits containing 1.00 g·kg^(-1) Ca^(2+).The laccase activity in O.formosanus body and cellulase activity in new-built fungus combs improved significantly,but the laccase activity in new-built fungus combs and cellulase activity in O.formosanus body reduced significantly after feeding baits containing 1.00 g·kg^(-1) Fe^(

关 键 词：黑翅土白蚁 菌圃 金属离子 酶活性 

分 类 号：S186[农业科学—农业基础科学]