Fgf8^(P2A-3×GFP/+):A New Genetic Mouse Model for Specifically Labeling and Sorting Cochlear Inner Hair Cells  

作　　者：Yi Pan Shuting Li Shunji He Guangqin Wang Chao Li Zhiyong Liu Mingliang Xiang 

机构地区：[1]Department of Otolaryngology and Head and Neck Surgery,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200025,China [2]Institute of Neuroscience,State Key Laboratory of Neuroscience,CAS Center for Excellence in Brain Science and Intelligence Technology,Chinese Academy of Sciences,Shanghai,200031,China [3]University of Chinese Academy of Sciences,Beijing,100049,China [4]Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology,Shanghai,201210,China

出　　处：《Neuroscience Bulletin》2023年第12期1762-1774,共13页神经科学通报（英文版）

基　　金：funded by the National Key R&D Program of China(2021YFA1101804);the Strategic Priority Research Program of the Chinese Academy of Science(XDB32060100);the National Natural Science Foundation of China(91949119,82101212,and 82101209);a Shanghai Municipal Science and Technology Major Project(2018SHZDZX05);the Science and Technology Commission of Shanghai Municipality(21ZR1440200);the Shanghai Sailing Program(20YF1426400).

摘　　要：The cochlear auditory epithelium contains two types of sound receptors,inner hair cells(IHCs)and outer hair cells(OHCs).Mouse models for labelling juvenile and adult IHCs or OHCs exist;however,labelling for embryonic and perinatal IHCs or OHCs are lacking.Here,we generated a new knock-in Fgf8^(P2A-3×GFP/+)(Fgf8^(GFP)/+)strain,in which the expression of a series of three GFP fragments is controlled by endogenous Fgf8 cis-regulatory elements.After confirming that GFP expression accurately reflects the expression of Fgf8,we successfully obtained both embryonic and neonatal IHCs with high purity,highlighting the power of Fgf8^(GFP)/+.Furthermore,our fate-mapping analysis revealed,unexpectedly,that IHCs are also derived from inner ear progenitors expressing Insm1,which is currently regarded as an OHC marker.Thus,besides serving as a highly favorable tool for sorting early IHCs,Fgf8^(GFP)/+will facilitate the isolation of pure early OHCs by excluding IHCs from the entire hair cell pool.

关 键 词：Fgf8 Inner Hair Cell Inner Ear-Cochlea 

分 类 号：R764[医药卫生—耳鼻咽喉科]