Whole-brain Optical Imaging:A Powerful Tool for Precise Brain Mapping at the Mesoscopic Level  

作　　者：Tao Jiang Hui Gong Jing Yuan 

机构地区：[1]Huazhong University of Science and Technology-Suzhou Institute for Brainsmatics,Jiangsu Industrial Technology Research Institute,Suzhou,215123,China [2]Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology,Wuhan,430074,China

出　　处：《Neuroscience Bulletin》2023年第12期1840-1858,共19页神经科学通报（英文版）

基　　金：supported by the STI2030-Major Projects(2021ZD0201001 and 2021ZD0201000);the National Natural Science Foundation of China(81827901 and 32192412).

摘　　要：The mammalian brain is a highly complex network that consists of millions to billions of densely-interconnected neurons.Precise dissection of neural circuits at the mesoscopic level can provide important structural information for understanding the brain.Optical approaches can achieve submicron lateral resolution and achieve“optical sectioning”by a variety of means,which has the natural advantage of allowing the observation of neural circuits at the mesoscopic level.Automated whole-brain optical imaging methods based on tissue clearing or histological sectioning surpass the limitation of optical imaging depth in biological tissues and can provide delicate structural information in a large volume of tissues.Combined with various fluorescent labeling techniques,whole-brain optical imaging methods have shown great potential in the brain-wide quantitative profiling of cells,circuits,and blood vessels.In this review,we summarize the principles and implementations of various whole-brain optical imaging methods and provide some concepts regarding their future development.

关 键 词：Whole-brain optical imaging Optical sectioning Micrometer resolution Brain connectome Neural circuits Neuron 

分 类 号：R338[医药卫生—人体生理学]