黄芩苷体外抗IBV QX株的作用研究  

Effects of baicalin against IBV QX strain in vitro

作　　者：郭霄慧 李伟欣 冯婉莹 赵鸿洁 李卫晴 王转转 陈光明 韩振兴 王冬喜 贾青辉[1] GUO Xiaohui;LI Weixin;FENG Wanying;ZHAO Hongjie;LI Weiqing;WANG Zhuanzhuan;CHEN Guangming;HAN Zhenxing;WANG Dongxi;JIA Qinghui(Hebei Province Key Laboratory of Preventive Veterinary Medicine,Hebei Normal University of Science and Technology,Qinhuangdao 066604,China;Hebei Muan Animal Pharmaceutical Co.,Ltd.,Shijiazhuang 050000,China;Hebei Province Broiler Breeding and Processing Technology Innovation Center,Chengde 067000,China;Hebei Luanping Huadu Food Co.,Ltd.,Chengde 067000,China)

机构地区：[1]河北科技师范学院,河北省预防兽医学重点实验室,河北秦皇岛066604 [2]河北牧安动物药业有限公司,石家庄050000 [3]河北省肉鸡养殖与加工技术创新中心,河北承德067000 [4]河北滦平华都食品有限公司,河北承德067000

出　　处：《黑龙江畜牧兽医》2024年第1期96-102,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：河北省现代农业产业技术体系蛋鸡产业创新团队岗位项目(HBCT2013090203);河北省重点研发计划项目(21326614D)。

摘　　要：为研究黄芩苷体外抗禽传染性支气管炎病毒(Avian infectious bronchitis virus,IBV)QX株的效果,试验对IBV进行复壮与扩繁,制备原代鸡胚肾(CEK)细胞,并测定IBV对CEK细胞的TCID50和黄芩苷对CEK细胞的最大无毒浓度(MNTC);在MNTC基础上采用CCK法测定不同浓度黄芩苷对IBV的有效抑制率,观察不同浓度黄芩苷对IBV导致的CEK细胞的细胞病变效应(CPE)的抑制情况,并采用qPCR法测定不同浓度黄芩苷对IBV N基因相对表达量的影响。结果表明:成功复壮并扩繁了IBV,其未被其他病毒污染,对CEK细胞的TCID50为1×10^(-4.75)/0.1 mL;黄芩苷对CEK细胞的MNTC为9.75 mg/L;该浓度黄芩苷对IBV的有效抑制率最高,为64.03%,能有效减轻IBV对CEK细胞的CPE;各浓度黄芩苷均可以极显著降低CEK细胞中IBV N基因相对表达量(P<0.01)。说明黄芩苷对CEK细胞的MNTC为9.75 mg/L,该浓度黄芩苷具有较好的体外抗IBV QX株的作用。In order to study the effects of baicalin against Avian infectious bronchitis virus(IBV)QX strain in vitro,IBV was rejuvenated and propagated.Primary chicken embryonic kidney(CEK)cells were prepared,and the TCID50 of CEK cells was determined by IBV.The maximum non-toxic concentration(MNTC)of baicalin on CEK cells was determined.On the basis of MNTC,the effective inhibition rate of different concentrations of baicalin on IBV was determined by CCK method,and the inhibition of different concentrations of baicalin on cytopathie effect(CPE)of CEK cells caused by IBV was observed.The effect of different concentrations of baicalin on the relative expression of IBV N gene was determined by qPCR.The results showed that IBV was successfully rejuvenated and propagated,and it was not contaminated by other viruses.The TCID50 of CEK cells was 1×10^(-4.75)/0.1 mL,and the MNTC of baicalin on CEK cells was 9.75 mg/L.The effective inhibition rate of baicalin at this concentration on IBV was the highest,which was 64.03%,which could effectively reduce the CPE of IBV on CEK cells.Baicalin at each concentration could significantly reduce the relative expression of IBV N gene in CEK cells(P<0.01).The results indicated that the MNTC of baicalin on CEK cells was 9.75 mg/L,and the concentration of baicalin had a good anti-IBV QX strain effect in vitro.

关 键 词：黄芩苷 禽传染性支气管炎病毒 鸡胚肾(CEK)细胞 最大无毒浓度 有效抑制率 

分 类 号：S859.3[农业科学—临床兽医学] S852.659.6[农业科学—兽医学]