2001—2022年鸽圆环病毒的遗传变异分析  

Analysis of the genetic variation in Pigeon circovirus from 2001 to 2022

作　　者：郎雯 陈煜杰 吴敏慧 苏梦容 阮莲 吕其壮 LANG Wen;CHEN Yujie;WU Minhui;SU Mengrong;RUAN Lian;LYU Qizhuang(College of Biology&Pharmacy,Yulin Normal University,Yulin 537000,China;Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology,Yulin 537000,China)

机构地区：[1]玉林师范学院生物与制药学院,广西玉林537000 [2]广西农产资源化学与生物技术重点实验室,广西玉林537000

出　　处：《黑龙江畜牧兽医》2024年第1期109-116,共8页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31860708);玉林师范学院大学生创新创业训练计划项目(202210606027)。

摘　　要：鸽圆环病毒(Pigeon circovirus,PiCV)是近几年发现的一种能引起感染鸽免疫抑制的病毒,属于圆环病毒科圆环病毒属。为研究PiCV的流行特征和遗传进化规律,试验从GenBank中选取208株PiCV全基因组序列,利用生物信息学软件,通过构建PiCV全基因组系统发育树,进行核苷酸同源性比较、氨基酸序列比对分析、抗原表位分析和基因重组等方式对其进行遗传变异分析。结果表明:基于构建的系统发育树可将208株PiCV毒株分为A(54.81%,114/208)和B(45.19%,94/208)两个基因型,其中A基因型可进一步分为A-1、A-2、A-3、A-4、A-55个不同的亚群,B基因型可进一步分为B-1、B-2、B-3、B-44个不同的亚群。208株PiCV全基因组序列的相似性为83.8%~100%。PiCV Cap蛋白氨基酸序列上存在氨基酸的点突变、缺失和插入等情况。208株PiCV全基因组序列中存在大量重组事件(44个),涉及A基因型序列中所包含的遗传物质的直接交换及ORF C1基因小片段的转移。说明PiCV的变异具有多样性。Pigeon circovirus(PiCV)is a virus found in recent years that can cause immunosuppression in infected pigeons.It belongs to the genus Circovirus of the circoviridae family.In order to study the epidemic characteristics and genetic evolution of PiCV,the whole genome sequences of 208 PiCV strains were selected from GenBank database and bioinformatics software was used in the experiment.The genetic variation of PiCV was analyzed by constructing phylogenetic tree,comparing nucleotide homology,amino acid sequence alignment,antigen epitope analysis and gene recombination.The results showed that 208 PiCV strains could be divided into two genotypes A(54.81%,114/208)and B(45.19%,94/208)based on the constructed phylogenetic tree,and the A genotype could be further divided into five different subpopulations A-1,A-2,A-3,A-4 and A-5.The B genotype could be further divided into four distinct subgroups:B-1,B-2,B-3 and B-4.The similarity of the whole genome sequence of 208 PiCV strains was 83.8%-100%.There were point mutation,deletion and insertion of amino acids in PiCV Cap protein.There were a large number of recombination events(44)in the whole genome sequence of 208 PiCV strains,involving the direct exchange of genetic material contained in the a genotype sequence and the transfer of small fragments of ORFC1 gene.It shows that the variation of PiCV was diverse.

关 键 词：鸽圆环病毒(PiCV) 遗传变异分析 重组分析 系统发育树 氨基酸序列分析 

分 类 号：S851.3[农业科学—预防兽医学]