综合化学实验:DNA 8-分支纳米结构的制备及表征  

Comprehensive Chemical Experiment:Preparation,Characterization of DNA 8-Branched Nanostructure

作　　者：孙四娟[1] 赵家婷 袁云兰[1] 雷鑫 段金伟[1] SUN Si-Juan;ZHAO Jia-Ting;YUAN Yun-Lan;LEI Xin;DUAN Jin-Wei(College of Sciences,Chang'an University,Xi'an 710064,China;Shaanxi Weishinuo Biotechnology Co.Ltd,Xi'an 710304,China)

机构地区：[1]长安大学理学院,陕西西安710064 [2]陕西维世诺生物科技有限公司,陕西西安710304

出　　处：《化学教育（中英文）》2024年第2期59-65,共7页Chinese Journal of Chemical Education

基　　金：中央高校基本科研业务费专项(300102121104);2021年教育部产学协同育人项目(202102448002);2021年长安大学高等教育教学改革研究项目(BY202140)。

摘　　要：借助DNA分子良好的生物相容性和可编程性,科学家合成了各种各样的DNA纳米自组装结构,这些结构在分子检测、生物成像和药物运输等领域已经展现出极大的应用潜力,然而这一先进前沿研究领域在本科生教学实验中并未涉及。为了拓展学生的科研视野,激发学生科研兴趣,将DNA纳米技术的科研成果与PCR、紫外-可见分光光度计、聚丙烯酰胺凝胶电泳和凝胶成像分析相融合,开展了DNA纳米自组装结构的教学实验。通过本实验使学生了解了DNA分支纳米结构的制备、表征和检测,不仅丰富了本科生实验教学项目的内容,培养了学生创新思维能力,提升了学生的科研水平,而且扩宽了学生的眼界、激发了学生进行前沿科学研究的热情,对培养复合型人才具有非常重要的意义。Scientists have synthesized a variety of DNA nano self-assembled structures by virtue of the good biocompatibility and programmability of DNA molecules,which have shown great application potential in the fields of molecular detection,biological imaging and drug deliv-ery.Obviously,it is the right time to conduct DNA nanotechnology teaching exploration and ex-perimental techniques training in undergraduate experiments.In this paper,DNA nanotechnolo-gy was combined with PCR,UV-vis spectrophotometer,polyacrylamide gel electrophoresis(PAGE)and gel imaging analysis.The experiment teaching can help students understand the basic of synthesis and detection method of DNA branched structures.To feedback experiment teaching by research achievements not only enrich the depth and breadth of the experiment teach-ing,but also broaden the students'scientific research field of vision,cultivate students'innova-tive thinking and improve the students'scientific research ability,which is of great significance to cultivate inter-disciplinary talent.

关 键 词：DNA分支结构 PCR 紫外-可见分光光度计 聚丙烯酰胺凝胶电泳 凝胶成像 

分 类 号：O6-3[理学—化学] G642.423[文化科学—高等教育学]