MRI质子密度脂肪分数评估限时饮食对高脂饮食诱导肥胖大鼠骨髓脂肪的影响  

Assessment of the effect of time-restricted feeding on bone marrow fat in high-fat diet-induced obese rats based on MRI proton density fat fraction

作　　者：丁煜堃 祝翠玲 张晓东 Ding Yukun;Zhu Cuiling;Zhang Xiaodong(Department of Medical Imaging,the Third Affiliated Hospital of Southern Medical University,Academy of Orthopedics Guangdong Province,Guangzhou 510630,China)

机构地区：[1]南方医科大学第三附属医院、广东省骨科研究院影像科,广州510630

出　　处：《中华放射学杂志》2024年第1期79-84,共6页Chinese Journal of Radiology

基　　金：国家自然科学基金(81801653);南方医科大学第三附属医院院长基金(YM2021012)。

摘　　要：目的采用MRI质子密度脂肪分数(PDFF)探讨限时饮食(TRF)对高脂饮食(HFD)诱导肥胖大鼠股骨近端骨髓脂肪的影响。方法将30只雄性Sprague-Dawley大鼠按照体重分层随机抽样分为6个亚组,每亚组5只,2个亚组合并为1组,共分为3组。对照组大鼠可24 h任意进食,饲喂正常饲料;HFD组大鼠可24 h任意进食,饲喂高脂饲料;HFD+TRF组大鼠只在9点(光照后2 h)至17点(黑暗前2 h)饲喂高脂饲料。实验第28天每组选取1个亚组的5只大鼠进行股骨MRI,第56天时对每组的另1个亚组进行扫描,基于mDixon-Quant定量序列图像,测量股骨近端骨髓脂肪的PDFF值。扫描结束后处死大鼠,采集血样,测量血清瘦素含量。采用单因素方差分析或Kruskal-Wallis H检验比较3组大鼠体重、PDFF和血清瘦素的差异,多重比较采用LSD-t检验。结果实验开始后第28天,3组大鼠间体重、PDFF和血清瘦素差异均无统计学意义(P>0.05)。第56天时,对照组、HFD组和HFD+TRF组大鼠股骨近端骨髓PDFF分别为(7.2±1.4)%、(9.7±2.4)%、(11.2±3.6)%,3组大鼠间体重、PDFF和血清瘦素差异均有统计学意义(F=6.95,P=0.010;F=5.98,P=0.007;F=4.54,P=0.034)。多重比较结果显示,HFD组的体重高于对照组(LSD-t=52.96,P=0.036)和HFD+TRF组(LSD-t=82.74,P=0.003);HFD+TRF组大鼠股骨近端骨髓PDFF值高于对照组(LSD-t=4.01,P=0.012);HFD组大鼠血清瘦素高于对照组(LSD-t=1.45,P=0.030)和HFD+TRF组(LSD-t=1.62,P=0.018)。结论TRF对HFD诱导肥胖大鼠减重的同时,股骨近端骨髓PDFF值增加,且可能与血清瘦素的降低有关。Objective To investigate the effect of time-restricted feeding(TRF)on bone marrow fat of proximal femur in obese rats induced by high-fat diet(HFD)using proton density fat fraction(PDFF).Methods Totally 30 male Sprague-Dawley rats were stratified and randomly sampled into 6 subgroups according to body weight,with 5 rats each.Then 2 subgroups were combined into one group,and there were totally 3 groups.The rats in the control group were fed with normal diet,and the rats could eat as much as they wanted for 24 h;the rats in the HFD group were fed with high-fat diet,and the rats could eat as much as they wanted for 24 h;the rats in the HFD+TRF group were fed with high-fat diet only between 9 AM(2 h after light)and 17 PM.One subgroup of rats from each group was examined with MRI on the femur on day 28 of the experiment,and the other subgroup from each group was examined on day 56 to measure the bone marrow PDFF of proximal femur based on mDixon-Quant quantitative sequence images.The rats were executed at the end of the scanning period,and blood samples were collected to measure serum levels of leptin.One-way ANOVA or Kruskal-Wallis H test was used to compare the differences in body weight,PDFF,and serum levels of leptin among 3 groups.The LSD-t test was used for multiple comparisons.Results On day 28 of the experiment,the differences in body weight,PDFF,and serum leptin among the 3 groups of rats were not statistically significant(P>0.05).On day 56,the bone marrow PDFF of proximal femur of the rats in the control group,HFD group,and HFD+TRF group were(7.2±1.4)%,(9.7±2.4)%,and(11.2±3.6)%,respectively.The differences in body weight,PDFF,and serum levels of leptin among the 3 groups of rats were statistically significant(F=6.95,P=0.010,F=5.98,P=0.007,F=4.54,P=0.034).The results of multiple comparisons showed that the body weight in the HFD group was higher than those in the control group(LSD-t=52.96,P=0.036)and the HFD+TRF group(LSD-t=82.74,P=0.003).The values of bone marrow PDFF of proximal femur in the HFD+TRF grou

关 键 词：磁共振成像 限时饮食 骨髓脂肪 肥胖 质子密度脂肪分数 动物实验 

分 类 号：R589.2[医药卫生—内分泌]