饲粮添加芦丁对断奶仔猪回肠形态结构和屏障功能的影响  

Effects of dietary rutin on the morphology and barrier function of ileum in weaned piglets

作　　者：马龙飞 周斌斌 刘春雨 陈顺 王恬[1] 王超[1] MA Longfei;ZHOU Binbin;LIU Chunyu;CHEN Shun;WANG Tian;WANG Chao(College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学动物科技学院,江苏南京210095

出　　处：《畜牧与兽医》2024年第2期7-14,共8页Animal Husbandry & Veterinary Medicine

基　　金：江苏省苏北科技计划项目(SZ-YC202146);国家自然科学基金项目(31972598)。

摘　　要：旨在研究饲粮添加芦丁对断奶仔猪肠道形态结构、黏膜屏障、抗氧化功能、炎症和细胞凋亡等方面的影响。试验选取28日龄健康且体重相近的断奶仔猪16头,随机分为2组,每组8个重复,每个重复1头猪。对照组饲喂基础饲粮,试验(芦丁)组在基础饲粮中添加500 mg/kg芦丁,试验期14 d。结果表明:与对照组相比,饲粮添加500 mg/kg芦丁对仔猪回肠形态结构无显著影响(P>0.05),但显著提高了仔猪回肠黏膜中过氧化氢酶(CAT)活性(P<0.05),显著提高了B细胞淋巴瘤基因-2(Bcl-2)、超氧化物歧化酶1(SOD1)、超氧化物歧化酶2(SOD2)以及闭合小环蛋白-1(ZO-1)mRNA的表达量(P<0.05),显著提高了闭合蛋白(Occludin)mRNA和蛋白的表达量(P<0.05),显著降低了肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)mRNA的表达量(P<0.05)。结果提示:饲粮添加500 mg/kg芦丁可提高断奶仔猪抗氧化能力和肠屏障功能,减少炎症发生和细胞凋亡,这可能是其抑制断奶仔猪腹泻的重要原因之一。The aim of this study was to determine the effects of dietary rutin on the morphology,mucosal barrier,antioxidant function,inflammation and apoptosis of the intestines in weaned piglets.Sixteen 28-day-old healthy weaned piglets with similar body weight were selected and randomly divided into 2 groups with 8 replicates per group and 1 pig per replicate.The piglets in the control group were fed with a basal diet,and those in the rutin group were fed with basal diet supplemented with 500 mg/kg rutin.The experiment lasted for 14 days.The results showed as follow:Compared with the piglets of the control group,dietary 500 mg/kg rutin had no significant effect on the morphology of the ileum of the piglets of the rutin group(P>0.05),but significantly increased the catalase(CAT)activity of their ileum mucosa(P<0.05).The mRNA expression of B-cell lymphoma gen-2(Bcl-2),superoxide dismutase 1(SOD1),superoxide dismutase 2(SOD2)and occlusive small loop protein-1(ZO-1)in the ileal mucosa of the experimental piglets was significantly increased(P<0.05),and the mRNA and protein expression of Occludin in the piglets was significantly increased(P<0.05)by the supplementary dietary rutin,while the mRNA expression levels of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in ileal mucosa of the piglets were significantly reduced(P<0.05).These results suggested that dietary 500 mg/kg rutin improved antioxidant capacity and intestinal barrier function of weaned piglets,and alleviated inflammation and apoptosis in them,which might be one of the important reasons for inhibiting diarrhea in weaned piglets by rutin.

关 键 词：芦丁 断奶仔猪 肠道形态 抗氧化 黏膜屏障 

分 类 号：S852[农业科学—基础兽医学]