新型鹅细小病毒徐州分离株NGPV XZ-01的分离和致病性研究  

Isolation and pathogenicity of the novel goose parvovirus Xuzhou strain NGPV XZ-01

作　　者：迟兰[1] 蔺辉星[2] CHI Lan;LIN Huixing(Xuzhou Vocational College of Bioengineering,Xuzhou 221006,China;Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]徐州生物工程职业技术学院,江苏徐州221006 [2]南京农业大学,江苏南京210095

出　　处：《畜牧与兽医》2024年第2期82-88,共7页Animal Husbandry & Veterinary Medicine

基　　金：徐州市2021年现代农业面上项目(KC21144);江苏省高等学校自然科学研究项目(20KJB230009);江苏省教育厅2022年江苏高校“青蓝工程”优秀青年骨干教师培养项目([2022]29)。

摘　　要：为了研究2021年6月江苏徐州某商品半番鸭养殖场所发疾病的病原及其致病性,从该养殖场发病的半番鸭群中无菌采集肝脏、脾脏、肠道等临床样品,采用PCR方法对病原进行鉴定,将所采集样本处理后接种至非免疫鸭胚中,连续多次传代后,对鸭胚组织及尿囊液进行病理学、电镜检查及动物试验。结果显示:PCR检测结果鉴定为新型鹅细小病毒阳性;鸭胚尿囊液在电镜下观察到细小病毒粒子,胚体病理切片观察显示符合细小病毒所致病变;动物试验显示,攻毒试验鸭主要表现为生长发育明显受阻,大部分试验鸭成为“僵鸭”,于20日龄后陆续出现上下喙变短、舌头外露等症状,剖检病变主要为肠内容物稀薄及泄殖腔膨大,肠道组织切片观察显示肠绒毛上皮细胞坏死、脱落及部分肠腺坏死,符合鸭短喙侏儒综合征(short beak and dwarfism syndrome,SBDS)所致雏鸭的症状与病变。结果表明:该肉鸭养殖场所发疾病为SBDS,将该场分离的病毒命名为新型鹅细小病毒徐州分离株(NGPV XZ-01株)。In order to determine the pathogen and pathogenicity of the disease in commercial semi-muscovy duck breeding facility in Xuzhou,Jiangsu Province,in June 2021,clinical samples of liver,spleen and intestines of the ducks were collected aseptically from half of the infected muscovy ducks in a farm.After nucleic acid extraction,the pathogens were identified using the PCR method.After processing,the collected samples were inoculated into non-immune duck embryos.The duck embryo tissue and allantoic fluid were examined by pathology,electron microscope and animal tests.The results showed that the novel goose parvovirus was identified by PCR,parvovirions were observed in the duck embryo allantoic fluid under the electron microscope,and pathological examination of the embryo body showed that the lesions were consistent with the parvovirus.The animal experiments showed that the main symptoms of the challenged ducks were that their growth and development were obviously hindered,and most of the tested ducks became“stiff ducks”.Their upper and lower beaks became shorter and their tongues were exposed successively after 20 days age of the test ducks.The lesions were mainly thin the intestinal contents and cloaca enlargement;and the intestinal tissue sections showed necrosis and exfoliation of the intestinal villous epithelial cells and necrosis of some intestinal glands.There were consistent with the symptoms and lesions of ducklings caused by SBDS.The present results indicated that the disease was SBDS.The virus isolated from the ducks in the farm was named the Xuzhou strain of novel goose parvovirus(NDPV XZ-01 strain).

关 键 词：鸭短喙侏儒综合征 新型鹅细小病毒 徐州分离株 分离鉴定 动物试验 

分 类 号：S855[农业科学—临床兽医学]