高压电击伤小鼠的血液代谢组学研究  

Metabonomic study of blood of mice with high-voltage electrical injury

作　　者：陈思羽 王徽 罗燕 陶嘉雯 张文娟 岳洋 余争平[1] 皮会丰 CHEN Si-yu;WANG Hui;LUO Yan;TAO Jia-wen;ZHANG Wen-juan;YUE Yang;YU Zheng-ping;PI Hui-feng(Military Labor Hygiene Teaching and Research Section,Department of Military Preventive Medicine,Army Medical University/Key Laboratory of Electromagnetic Radiation Medical Protection of Ministry of Education,Chongqing 400038,China)

机构地区：[1]陆军军医大学军事预防医学系军队劳动卫生学教研室/教育部电磁辐射医学防护重点实验室,重庆400038

出　　处：《局解手术学杂志》2024年第2期100-106,共7页Journal of Regional Anatomy and Operative Surgery

基　　金：重庆市科卫联合医学科研项目(重大项目)(2023DBXM006)。

摘　　要：目的探讨暴露于高压电击后小鼠的血液代谢组学变化,并筛选出其中显著改变的差异代谢物和代谢通路。方法将C57BL/6J小鼠头部进行高压电击处理(电击组)和相同电击装置的声光刺激暴露(对照组)后,取全血并分离出血清,进行基于气相色谱-质谱(GC-MS)/液相色谱-反谱(LC-MS)的双平台联用代谢组学分析,采用正交偏最小二乘法(OPLS-DA)模型筛选其差异代谢物和相关代谢通路。结果高压电击后小鼠的血液代谢组学筛选出415种差异代谢物,包含上调187种,下调228种;这些差异代谢物被显著富集到的代谢通路包括癌症中心碳代谢、胰高血糖素信号通路等。结论本研究通过建立实验小鼠高压电击伤模型,揭示高压电击伤引起血液中代谢物含量和代谢通路的显著变化,为高压电击伤损害血液代谢活动提供了依据,提示高压电击伤对全身血液代谢活动的潜在危害。Objective To explore the changes of metabonomics in blood of mice after high-voltage electric shock,then screen out the significantly changed differential metabolites and metabolic pathways.Methods The head of C57BL/6J mice was subjected to high-voltage electric shock(electric shock group)or exposed to acoustic and optical stimulation of high-voltage electric(control group),then the whole blood from mice were collected to separate serum.The dual platform combined metabonomic analysis based on gas chromatography-mass spectrometer(GC-MS)and liquid chromatography-mass spectrometer(LC-MS)was performed and orthogonal partial least squares discriminant analysis(OPLS-DA)was used to screen the differential metabolites and related metabolic pathways.Results A total of 415 differential metabolites were screened out in metabonomics in blood of mice after high-voltage electric shock,including 187 up-regulated and 228 down-regulated metabolites.These differentially metabolites were significantly enriched in metabolic pathways including central carbon metabolism in cancer,glucagon signaling pathway,etc.Conclusion By establishing the model of high-voltage electrical injury on experimental mice,this study reveals the significant change of metabolite content and metabolic pathway in blood by high-voltage electrical injury.Which provides a basis for the damage of blood metabolic activity by high-voltage electrical injury,and suggests the potential harm of high-voltage electrical injury to blood metabolic activity in the whole body.

关 键 词：电工作业 高压电击伤 血液 血生化 代谢组学 

分 类 号：R135.99[医药卫生—劳动卫生]