姜黄素对糖尿病小鼠皮肤创面愈合的影响  

Effect of Curcumin on Promoting Skin Wound Healing in Diabetes Mice

作　　者：张津 崔新刚 朱彦兆 苏梦 贝颖 黄玉 李美运 武艳[1] ZHANG Jin;CUI Xingang;ZHU Yanzhao;SU Meng;BEI Ying;HUANG Yu;LI Meiyun;WU Yan(College of Life Sciences,Mudanjiang Medical College,Mudanjiang 157000,China;Department of Nutrition and Food Hygiene,School of Public Health,Mudanjiang Medical College,Mudanjiang 157000,China;Wenzhou Research Institute of Shanghai University,Wenzhou 325000,China)

机构地区：[1]牡丹江医学院生命科学学院,牡丹江157000 [2]牡丹江医学院公共卫生学院营养与食品卫生学教研室,牡丹江157000 [3]上海大学温州研究院,温州325000

出　　处：《医药导报》2024年第2期167-174,共8页Herald of Medicine

基　　金：黑龙江省自然科学基金联合引导项目(LH2020H076)。

摘　　要：目的研究姜黄素对糖尿病小鼠创面愈合的影响。方法采用噻唑蓝(MTT)检测姜黄素对成纤维细胞活性影响。氧自由基(ROS)检测试剂盒检测姜黄素对过氧化氢诱导的成纤维细胞中ROS的清除效果。qRT-PCR检测姜黄素对脂多糖诱导RAW 264.7巨噬细胞炎症因子(CD86、IL-6、CD206及ARG1)mRNA表达影响。腹腔注射链脲佐菌素建立糖尿病创面模型。苏木精-伊红(HE)、马松(Masson)染色评估创面愈合与组织形态学变化,免疫荧光染色法测定皮肤组织α-平滑肌肌动蛋白、CD86、CD206表达。结果姜黄素在浓度<20 mol·L^(-1)对成纤维细胞活性没有显著影响,并可清除过氧化氢诱导的成纤维细胞内的ROS;姜黄素降低脂多糖诱导的RAW 264.7巨噬细胞CD86、IL-6 mRNA表达量,同时增加CD206、ARG1 mRNA表达量。体内给药后,与对照组比较,姜黄素(15、30 mg·mL^(-1))组伤口闭合率加快(P<0.01),肉芽组织明显增加,胶原蛋白的沉积明显增多,CD206的表达明显增高(P<0.01),CD86的表达明显减少(P<0.01),且姜黄素(30 mg·mL^(-1))组伤口中平滑肌α-肌动蛋白表达量较小剂量姜黄素组明显增加(P<0.01)。结论姜黄素通过抗炎、抗氧化作用,促进糖尿病小鼠皮肤创面的肉芽组织增生和胶原沉积,进而加快糖尿病创面的愈合。Objective To study the effect of curcumin on wound healing in diabetic mice.Methods The effect of curcumin on fibroblast activity was examined by the MTT assay,and the ROS detection kit was used to detect the effect of curcumin on the hydrogen peroxide-induced scavenging effect of reactive oxygen species(ROS)in fibroblasts.Q-PCR was used to detect the effects of curcumin on the mRNA expression of inflammatory factors CD86,CD206,IL-6 and ARG1 in lipopolysaccharide-induced RAW 264.7 macrophage.The wound model of diabetes was established by intraperitoneal injection of streptozotocin.Hematoxylin-eosin(HE)and Masson staining were used to evaluate wound healing and histomorphological changes,and immunofluorescence staining was used to determine skin tissueα-smooth muscle actin,CD86 and CD206 expression.Results Curcumin had no significant effect on fibroblast activity at concentrations less than 20 mol·L^(-1);curcumin scavenged hydrogen peroxide-induced intracellular ROS in fibroblasts;curcumin decreased the mRNA expression of CD86 and IL-6 while increasing CD206 and ARG1 in lipopolysaccharide-induced RAW 264.7 macrophages.After in vivo administration,compared with the control group,wound healing was significantly faster in the curcumin(15,30 mg·mL^(-1))group after 7 d and 14 d of wound perforation(P<0.01).Hematoxylin-eosin(HE)and Masson staining results confirmed a significant increase in granulation tissue and a significant increase in collagen deposition in the curcumin(15,30 mg·mL^(-1))group.Immunofluorescence assay showed significantly higher expression of CD206(P<0.01)and significantly reduced expression of CD86(P<0.01)in the skin wounds of curcumin(15,30 mg·mL^(-1))for 14 d.In addition,the expression ofα-SMA in the wound of the high-dose curcumin(30 mg·mL^(-1))group was significantly higher than that of the low-dose curcumin group(P<0.01).Conclusion Curcumin accelerates diabetic wound healing by promoting granulation tissue proliferation and collagen deposition in refractory diabetic wounds in mice throug

关 键 词：姜黄素 抗炎 抗氧化 巨噬细胞 糖尿病动物模型 创面愈合 

分 类 号：R965[医药卫生—药理学]