Mechanism study of the effect of Ningxue Shengban decoction on regulating the ratio of Treg/Th17 cells and downstream inflammatory factors in immune thrombocytopenia model mice  

作　　者：Ai-Di Wang Hong-Lei Guo Dong-Jie Xie Xiang-Yu Zuo Lin Ma Bao-Shan Liu 

机构地区：[1]Department of Chinese Medicine,Tianjin Medical University General Hospital,Tianjin 300052,China [2]Graduate School,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China [3]Department of Oncology,the First Affiliated Hospital of Henan University of Science and Technology,Henan 471003,China [4]College of Traditional Chinese Medicine Health Service,Shanxi Datong University,Shanxi 037009,China

出　　处：《Integrative Medicine Discovery》2023年第36期1-7,共7页

基　　金：Supported by National Natural Science Foundation of China:Mechanism study of Xijiao Dihuang combined Prescription in Treating Immune Thrombocytopenia Based on PD-1/PD-L1 Regulating Teff/Treg Immune Balance(No.8200152588);National Natural Science Foundation of China:Study on the mechanism of Yiqi Yangyin Qingre Huayu Recipe regulation of ITP platelet destruction and production through the imbalance of Th17/Treg and Notch pathway(No.81373847).

摘　　要：Background:To explore the role and mechanism of T cell imbalance in the process of immune thrombocytopenic purpura(ITP)and the efficacy of the Ningxue Shengban decoction in treating this disease.Methods:Passive immune ITP mouse model was established by injecting CD41 monoclonal antibody into BALB/c mice.The mice were divided into different groups for intervention and drug administration for 9 days.The therapeutic effects(blood cell count,bone marrow morphology)were observed.The changes in the number and proportion of regulatory T/Th17 cells in each group,as well as the expression of key transcription proteins and genes(Foxp3,RORγt)in mouse spleen,and the secretion of related inflammatory factors(interleukin-17,TGF-β,interleukin-21,interleukin-10)in serum were detected.Results:Ningxue Shengban decoction significantly increased the peripheral blood platelet count in ITP mice,improved bone marrow morphology,restored the imbalance of regulatory T/Th17 ratio,and exerted a positive regulatory effect on target proteins and downstream inflammatory factor secretion.Conclusion:Ningxue Shengban decoction may exert its therapeutic effect in treating ITP by regulating T cells and exerting immune regulatory function.

关 键 词：immune thrombocytopenic purpura immunologic balance traditional Chinese medicine Ningxue Shengban decoction 

分 类 号：R285[医药卫生—中药学]