人胚神经干细胞定向诱导分化为多巴胺能神经元的实验研究  

The committed induction of dopaminergic neuron derived from human fetal neural stem cell by bone marrow stromal cell.

作　　者：张力[1] 江澄川[1,2] 徐斌 陈商群 冯林音[3] 江澄川 

机构地区：[1]复旦大学附属华山医院神经外科,上海200040 [2]国家自然科技基金资助课题负责人 [3]中国科学院神经科学研究所

出　　处：《中国神经精神疾病杂志》2002年第6期410-413,共4页Chinese Journal of Nervous and Mental Diseases

基　　金：国家自然科技基金资助课题(编号:39870745A)

摘　　要：目的　建立神经干细胞与骨髓基质细胞的共培养系统,根据该系统的条件性培养液诱导多巴胺能神经元的分化。方法　来源于胎脑海马、纹状体、额叶、中脑的神经干细胞与骨髓基质细胞建立起各自的共培养系统,并根据数种条件性培养液诱导神经干细胞的分化,以免疫细胞化学检测神经元的总体分化率及多巴胺能神经元的诱导率。结果　骨髓基质细胞及CO-BMSC能显著提高不同来源的神经干细胞的神经元分化率,同时只有中脑神经干细胞能被有效地进行多巴胺能神经元的诱导。结论 共培养系统诱发了神经干细胞与骨髓基质细胞的自/旁分泌作用,该作用可根据神经干细胞的区域特异性有效的定向诱导中脑神经干细胞的分化。Objective To establish a co-culture system with bone marrow stromal cell and human fetal neural stem cell, and induce the differentiation of neural stem cell into dopaminergic neuron. Methods The human fetal neural stem cell derived from hippocampus、striatum、front lobe、midbrain were purified and induced by different conditioned medium extrated from co-culture system, the induced differentiational rate of all neurons and dopaminergic neurons were detected by immunocytochemistry and im-munocytoflurescence respectively. Results The differentiational rate of neurons were induced greatly by BMSC and CO-CM. However, only midbrain neural stem cells were induced to differentiate into dopaminergic neurons efficiently. Conclusions The co-culture system induced the effect of the auto/paracrine exsisted in the BMSC and neural stem cell, it maby committedly induce the dopaminergic neurons based on the regional-speciafiction of midbrain neural stem cells.

关 键 词：人胚神经干细胞 定向诱导分化 实验研究 骨髓基质细胞 多巴胺能神经元 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]