电针对快速老化模型小鼠骨骼肌线粒体动力学的影响  

Effects of Electroacupuncture on Skeletal Muscle Mitochondrial Dynamics in SAMP8 Mice

作　　者：温若兰 郭婉清 董卫国[3] 陈丽敏 李金全 温辰光 林岚[3] 王丰[3] WEN Ruolan;GUO Wanqing;DONG Weiguo;CHEN Limin;LI Jinquan;WEN Chenguang;LIN Lan;WANG Feng(College of Acupuncture and Moxibustion,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;The Third Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine,Fuzhou 350108,China;College of Integrated Chinese and Western Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学针灸学院,福建福州350122 [2]福建中医药大学附属第三人民医院,福建福州350108 [3]福建中医药大学中西医结合学院,福建福州350122

出　　处：《中国中医药信息杂志》2024年第2期104-109,共6页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81973923);福建省自然科学基金(2022J01348);福建省中医药科研项目(2021zyjc23)。

摘　　要：目的观察电针对快速老化模型(SAMP8)小鼠运动功能及骨骼肌线粒体动力学的影响,从线粒体动力学角度探讨电针改善阿尔茨海默病(AD)运动功能障碍的作用机制。方法18只SAMP8小鼠随机分为模型组和电针组,每组9只,另9只同龄抗快速老化(SAMR1)小鼠作为对照组。电针组选取“百会”“大椎”“肾俞”,每日电针20 min,8 d为1个疗程,间隔2 d,共3个疗程,模型组和对照组不予干预。抓力测试、悬挂实验、后肢伸展实验和Morris水迷宫实验检测小鼠运动功能,HE染色观察腓肠肌组织形态,比色法检测腓肠肌组织三磷酸腺苷(ATP)含量,实时荧光定量PCR检测腓肠肌组织视神经萎缩症蛋白1(OPA1)、线粒体融合蛋白2(MFN2)和线粒体动力相关蛋白1(DRP1)mRNA表达,Western blot检测腓肠肌组织OPA1、MFN2和DRP1蛋白表达。结果与对照组比较,模型组小鼠抓力、悬挂实验评分及Morris水迷宫实验平均速度和最大速度显著降低(P<0.01);腓肠肌纤维排列混乱、间隙变宽、细胞核分布不均匀,腓肠肌组织ATP含量显著降低(P<0.01),OPA1、MFN2 mRNA和蛋白表达显著降低(P<0.01),DRP1 mRNA和蛋白表达显著升高(P<0.01)。与模型组比较,电针组小鼠抓力、悬挂实验评分及Morris水迷宫实验平均速度和最大速度显著升高(P<0.01);腓肠肌纤维排列较整齐、间隙变窄、细胞核分布较均匀,腓肠肌组织ATP含量显著升高(P<0.01),OPA1、MFN2 mRNA和蛋白表达显著升高(P<0.05,P<0.01),DRP1 mRNA和蛋白表达显著降低(P<0.01)。结论电针可改善SAMP8小鼠骨骼肌形态结构和运动功能障碍,其机制可能与调节骨骼肌线粒体动力学失衡状态,提高骨骼肌ATP含量有关。Objective To observe the effects of electroacupuncture on the motor function and mitochondrial dynamics of skeletal muscle of SAMP8 mice;To explore the mechanism of electroacupuncture in improving the motor dysfunction of Alzheimer disease(AD)from the perspective of mitochondrial dynamics.Methods Totally 18 SAMP8 mice were divided into model group and electroacupuncture group,with 9 mice in each group,and the SAMR1 mice with the same age were set as control group.“Baihui”,“Dazhui”and“Shenshu”were selected in the electroacupuncture group,and electroacupuncture was performed daily for 20 min,8 d as a course of treatment.Each course of treatment was separated by 2 d,for a total of 3 courses of treatment.The model group and the control group were not intervened.The motor function of mice was tested by grip strength test,suspension test,hind limb extension test and Morris water maze experiment.The morphology and structure of gastrocnemius were observed by HE staining,ATP content in gastrocnemius was determined by colorimetry,the mRNA expression of optic atrophy 1(OPA1),mitofusin 2(MFN2)and dynamin-related protein 1(DRP1)in gastrocnemius were detected by real-time quantitative PCR,the expressions of OPA1,MFN2 and DRP1 in gastrocnemius were detected by Western blot.Results Compared with the control group,the grip strength,the score in suspension test,and the average speed and maximum speed of Morris water maze experiment of mice in model group significantly decreased(P<0.01);the arrangement of fibers in the gastrocnemius muscle tissue was disordered,the gaps become wider,and the distribution of nuclei was uneven;the ATP content in the gastrocnemius muscle tissue was significantly decreased(P<0.01),the mRNA and protein expressions of OPA1 and MFN2 were significantly decreased(P<0.01),and the expression of DRP1 mRNA and protein significantly increased(P<0.01).Compared with the model group,the grip strength,the score in suspension test,and the average speed and maximum speed of Morris water maze experiment in e

关 键 词：阿尔茨海默病 电针 线粒体动力学 运动功能障碍 快速老化模型小鼠 

分 类 号：R245.3[医药卫生—针灸推拿学]