生长调节剂对马大杂种相思开放式组培增殖的影响  

Effects of Growth Regulators on Acacia mangium×A.auriculiformis Proliferation in Open Tissue Culture

作　　者：李泽瑞 卢艳平 陈玉军[1] 李玫[1] 黄烈健[1] LI Zerui;LU Yanping;CHEN Yujun;LI Mei;HUANG Liejian(Research Institute of Tropical Forestry,Chinese Academy of Forestry,Guangzhou,Guangdong 510520,China)

机构地区：[1]中国林业科学研究院热带林业研究所,广东广州510520

出　　处：《林业与环境科学》2023年第6期35-39,共5页Forestry and Environmental Science

基　　金：中央级公益性科研院所基本科研业务费专项基金(CAFYBB2017MB009)。

摘　　要：为实现马大杂种相思Acacia mangium×A.auriculiformis树种的开放组培增殖,建立马大杂种相思开放式组培增殖培养体系,文章以马大杂种相思组培苗为试验材料,在无蔗糖的条件下,采用正交设计研究生长调节剂6-BA、IBA、NAA对马大杂种相思芽增殖的影响。结果表明:不同质量浓度的6-BA、IBA、NAA对马大杂种相思的增殖有显著影响;马大杂种相思开放组培最佳增殖培养基为:MS+2.5mg·L^(-1)6-BA+0.1mg·L^(-1)IBA+1.0mg·L^(-1)IAA+0.2g·L^(-1)百菌清,增殖倍数为2.97。通过对不同生长调节剂的种类和浓度进行组合,可以在无蔗糖的培养基中实现马大杂种相思有效增殖。In order to realize the open tissue culture and proliferation of Acacia mangium×A.auriculiformis,an open tissue culture and proliferation cultrue system of A.mangium×A.auriculiformis was established.In this paper,A.mangium×A.auriculiformis were used as test materials.An orthogonal design was used to study the effects of growth regulators 6-BA,IBA,and NAA on the proliferation under the condition of no sucrose,aiming to establish A.mangium×A.auriculiformis open tissue culture proliferation culture systems.The results showed that different concentrations of 6-BA,IBA and NAA had significant effects on the proliferation of A.mangium×A.auriculiformis;the effects of different growth regulators on the proliferation of A.mangium×A.auriculiformis were as follows:IAA>6-BA>IBA.The best growth medium for open tissue culture is:MS+2.5 mg·L^(-1)6-BA+0.1 mg·L^(-1)IBA+1.0 mg·L^(-1)IAA+0.2 g·L^(-1)chlorothalonil,the multiple of proliferatione achieved 2.97.The research results lay an important foundation for the further establishment of a perfect open tissue culture system of Acacia,and also provide an important reference for the related research of other tree species.

关 键 词：马大杂种相思 开放式组织培养 生长调节剂 增殖 

分 类 号：S792.99[农业科学—林木遗传育种]