Large scale genetic landscape and population structure of Ethiopian sesame (Sesamum indicum L.) germplasm revealed through molecular marker analysis  

作　　者：Muez Berhe Jun You Komivi Dossa Fetien Abay Abera Emmanuel Amponsah Adjei Yanxin Zhang Linhai Wang 

机构地区：[1]Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,and Rural Afairs,No.2 Xudong 2nd Road,Wuhan,430062,China [2]Tigray Agricultural Research Institute,Humera Agricultural Research Center,P.O.Box 62,Tigray,Ethiopia [3]CIRAD,UMR AGAP Institut,97170 Petit Bourg,Guadeloupe,France [4]Department of Dryland Crop and Horticultural Sciences,College of Dryland Agriculture and Natural Resources,Mekelle University,Mekelle,Tigray,Ethiopia [5]University of California Davis Department of Plant Sciences,MS/11 Shields Ave,Davis,CA,95616,USA [6]CSIR-Savanna Agriculture Research Institute,P.O.Box TL52,Tamale,Ghana [7]UMR AGAP Institut,Univ Montpellier,CIRAD,INRAE,Institut Agro,F-34398 Montpellier,France

出　　处：《Oil Crop Science》2023年第4期266-277,共12页中国油料作物学报(英文版）

基　　金：funded by the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2021-OCRI);China Agriculture Research System(CARS14);Hubei International Science and Technology Cooperation Project(2022EHB034);the Science and Technology Innovation Project of Hubei Province(2021-620-000-001-035);the Fundamental Research Funds for Central Non-profit Scientific Institution(2013).

摘　　要：Sesame(Sesamum indicum L.) plays a crucial role in Ethiopian agriculture,serving both subsistence and commercial purposes.However,our understanding of the extensive genetic diversity and population structure of Ethiopian sesame remains limited.To address this knowledge gap,we genotyped 368 Ethiopian sesame germplasms,categorizing into four distinct breeding groups:Accessions,landraces,improved varieties,and wild types,using a comprehensive set of 28 polymorphic markers,including 23 simple sequence repeat(SSR) and five Insertion-Deletion(InDel) markers.These markers ensured robust genomic representation,with at least two markers per linkage group.Our results unveiled substantial genetic diversity,identifying a total of 535 alleles across all accessions.On average,each locus displayed 8.83 alleles,with observed and expected heterozygosity values of 0.30 and 0.36,respectively.Gene Diversity and Polymorphic Information Content(PIC) were recorded at 0.37 and 0.35.The percentage of polymorphic loci varied significantly among breeding groups,ranging from8.00% to 82.40%,indicating high diversity in accessions(82.4%),moderate diversity in improved varieties(31.20%) and landraces(29.60%),and limited diversity in wild types(8.00).Analysis of Molecular Variance(AMOVA) results emphasized significant genetic differentiation among populations,with substantial diversity(P<0.001) within each population.Approximately 8% of the entire genetic diversity could be attributed to distinctions among populations,while the larger proportion of genetic diversity(92%) resided within each individual sesame population,showcasing heightened diversity within each group.Our study’s findings received support from both Bayesian clustering and Neighbor-joining(NJ) analysis,reaffirming the credibility of our genetic structure insights.Notably,Population structure analysis at its highest Δk value(k=2) revealed the existence of two primary genetic clusters,further subdivided into four sub-populations at k=4.Similarly,NJ analysis identified two promi

关 键 词：Alleles Conservation Genetic diversity Molecular markers Population structure 

分 类 号：S565.3[农业科学—作物学]