几种诱抗剂对西瓜ZYMV的防治效果评价  

Evaluation of Several Resistance Inducers’Control Effects on ZYMV in Watermelon

作　　者：郝小苑 刘卓文 刘枫楠 刘莉铭[1] 吴会杰[1] 古勤生[1] 康保珊[1,2] HAO Xiaoyuan;LIU Zhuowen;LIU Fengnan;LIU Liming;WU Huijie;GU Qinsheng;KANG Baoshan(Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Henan Key Laboratory of Fruit and Cucurbit Biology,Zhengzhou 450009,Henan,China;Zhongyuan Research Center,Chinese Academy of Agricultural Sciences,Xinxiang 453500,Henan,China)

机构地区：[1]中国农业科学院郑州果树研究所,河南省果树瓜类生物学重点实验室,河南郑州450009 [2]中国农业科学院中原研究中心,河南新乡453500

出　　处：《中国蔬菜》2024年第1期60-67,共8页China Vegetables

基　　金：财政部和农业农村部:国家现代农业产业技术体系项目(CARS-25);中国农业科学院科技创新工程项目(CAAS-ASTIP-2022-ZFRI-09);国家自然科学基金项目(31701942)。

摘　　要：为探索对西瓜病毒病具有防效作用的植物免疫诱抗剂,以小西葫芦黄花叶病毒(zucchini yellow mosaic virus,ZYMV)为防治对象,植株的显症率及相对防效为指标,评价了6种不同浓度的哌啶酸(Pip)、N-羟基哌啶酸(NHP)、没食子酸、苯并噻二唑(BTH)、壳寡糖、β–氨基丁酸(β-aminobutyric acid,BABA)及其2种药剂和3种药剂复配后对该病毒的防治效果。结果显示,单一药剂喷施后对西瓜ZYMV的防效从高到低依次为:NHP(0.5 mmol·L^(-1))、Pip(2 mmol·L^(-1))、壳寡糖(1 g·L^(-1))、没食子酸(0.5 g·L^(-1))、BABA(1 mmol·L^(-1))、BTH(0.1 mmol·L^(-1));复配药剂试验中,2种药剂复配防效最好的是2-Ⅶ(NHP 0.5 mmol·L^(-1)+Pip 2 mmol·L^(-1)),接种后11 d、无菌水对照植株显症率达到100.00%时,该组合处理的植株显症率为10.00%,相对防效达89.99%,但该复配药剂会影响植株生长;3种药剂复配防效较好的组合是3-Ⅰ(没食子酸0.5 g·L^(-1)+NHP 0.5 mmol·L^(-1)+BABA 1 mmol·L^(-1))和3-Ⅱ(没食子酸0.5 g·L^(-1)+NHP 0.5 mmol·L^(-1)+Pip 2 mmol·L^(-1)),显症率分别为27.27%、33.33%,相对防效分别为79.49%、68.27%,显著高于其他组合,其中3-Ⅱ处理的植株显症率增加缓慢,防病效果较其他处理持久,防治效果最优。综上,NHP、Pip和没食子酸3种诱抗剂在预防西瓜病毒病害中具有潜在的应用价值。In order to explore plant immunoinducer with control effect to watermelon virus disease,taking zucchini yellow mosaic virus(ZYMV)as control object,plant pathogenicity and relative control efficiency as indicators,this paper evaluated 6 agents with different concentrations,including picolinic acid(Pip),N-hydroxy-picolinic acid(NHP),gallic acid,benzothiadiazole(BTH),chitosan,β-aminobutyric acid(BABA),and their two-agent and three-agent combinations for their control effects on this virus disease.The results showed that the control effect of single-agent on ZYMV in watermelon ranked from high to low as follows:NHP(0.5 mmol·L^(-1)),Pip(2 mmol·L^(-1)),chitosan(1 g·L^(-1)),gallic acid(0.5 g·L^(-1)),BABA(1 mmol·L^(-1)),BTH(0.1 mmol·L^(-1)).In the combination agent experiment,the best control effect of the two-agent combination was 2-Ⅶ(NHP 0.5 mmol·L^(-1)+Pip 2 mmol·L^(-1)).Eleven days after inoculation,when incidence rate of the plant by control-treated water reached 100.00%,the incidence rate of the combination-treated plants was 10.00%,and relative control effect was 89.99%.However,this combination agent would affect plant growth.The combination of 3 agents with better control effect were 3-Ⅰ(gallic acid 0.5 g·L^(-1)+NHP 0.5 mmol·L^(-1)+BABA 1 mmol·L^(-1))and 3-Ⅱ(gallic acid 0.5 g·L^(-1)+NHP 0.5 mmol·L^(-1)+Pip 2 mmol·L^(-1)).Their incidence rates were 27.27%and 33.33%,respectively,and their relative control effects were 79.49%and 68.27%,respectively,significantly higher than those of the other combinations.Among them,the incidence rate of the plants treated with 3-Ⅱincreased slowly,and disease control effect was more lasting than that of the other treatments.Thus,3-Ⅶhad the best control effect on ZYMV.The results indicate that NHP,Pip and gallic acid had potential application value in preventing watermelon virus diseases.

关 键 词：诱抗剂 西瓜 小西葫芦黄花叶病毒 防效 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]