氚水长期暴露对斑马鱼子代生长发育影响的研究  

Effects of long-term exposure to tritiated water on the growth and development of zebrafish offspring

作　　者：顾鹏诚 罗发坚 薛惠元 陈娜[1] 孙亮[1] 万骏[1] 崔凤梅 涂彧[1] Gu Pengcheng;Luo Fajian;Xue Huiyuan;Chen Na;Sun Liang;Wan Jun;Cui Fengmei;Tu Yu(State Key Laboratory of Radiation Medicine and Protection,School of Radiation Medicine and Protection,Suzhou Medical College of Soochow University,Suzhou 215123,China)

机构地区：[1]苏州大学苏州医学院放射医学与防护学院,放射医学与辐射防护国家重点实验室,苏州215123

出　　处：《国际放射医学核医学杂志》2023年第10期593-601,共9页International Journal of Radiation Medicine and Nuclear Medicine

基　　金：国家自然科学基金(U186720)。

摘　　要：目的研究氚水长期暴露对斑马鱼子代生长发育的影响。方法将野生型AB品系斑马鱼所产胚胎暴露在0、1×10^(2)、1×10^(5) Bq/L氚水中长期饲养作为亲代(F0代), 待其性成熟后进行繁殖, 所得子代作为F1代。F1代斑马鱼继续饲养在与F0代对应浓度的氚水中。观察F1代斑马鱼的生长发育情况, 检测胚胎期的自主运动、心率, 幼苗期的孵化率、体长、活性氧(ROS)荧光强度, 幼鱼期的总超氧化物歧化酶(T-SOD)、丙二醛(MDA)、总氚含量, 成鱼期的产卵量。各检测指标的组间比较采用t检验(方差齐)。结果 F1代0、1×10^(2)、1×10^(5) Bq/L氚水暴露组斑马鱼的孵化率分别为(90.66±0.05)%、(85.63±0.10)%、(78.06±0.15)%, 与0 Bq/L氚水暴露组相比, 1×10^(2)、1×10^(5) Bq/L氚水暴露组F1代斑马鱼孵化率的差异均无统计学意义(t= 0.785、1.370, P=0.462、0.220)。F1代3组斑马鱼受精后24 h的自主运动次数分别为(12.93± 2.70)、(11.30±0.78)、(10.50±0.80)次/min, 与0 Bq/L氚水暴露组相比, 1×10^(2)、1×10^(5) Bq/L氚水暴露组F1代斑马鱼受精后24 h自主运动次数的差异均无统计学意义(t=1.008、1.499, P=0.370、0.208)。3组斑马鱼受精后36 h的自主运动次数分别为(3.63±1.43)、(4.50±1.15)、(5.40±3.55)次/min, 与0 Bq/L氚水暴露组相比, 1×10^(2)、1×10^(5) Bq/L氚水暴露组F1代斑马鱼受精后36 h自主运动次数的差异均无统计学意义(t=0.817、0.799, P=0.460、0.469)。3组斑马鱼受精后48 h的心率分别为(59.43±6.93)、(65.00±3.30)、(61.23±4.55)次/20 s, 与0 Bq/L氚水暴露组相比, 1×10^(2)、1×10^(5) Bq/L氚水暴露组F1代斑马鱼受精后48 h心率的差异均无统计学意义(t=1.256、0.376, P=0.278、0.726)。3组斑马鱼受精后60 h的心率分别为(69.87±2.71)、(66.17± 6.97)、(69.77±9.08)次/20 s, 与0 Bq/L氚水暴露组相比, 1×10^(2)、1×10^(5) Bq/L氚水暴露组F1代斑马鱼受精后60 h心率的差异均无统计学意义(t=0.857、0.018, P=0Objective To study the effects of long-term tritiated water exposure on the growth and development of zebrafish offspring.Methods Embryos produced by wild-type AB strain zebrafish were exposed to 0,1×10^(2),and 1×10^(5) Bq/L tritiated water for long-term feeding as parents(F0 generation).After their sexual maturity,they reproduced,and the offspring obtained were recorded as F1 generation.The F1 generation zebrafish continued to be raised in tritiated water concentrations corresponding to the F0 generation.We observed the growth and development of F1 generation zebrafish and detected autonomous movement and heart rate during the embryonic stage;hatching rate,body length,and reactive oxygen species(ROS)fluorescence intensity during the seedling stage;total superoxide dismutase(T-SOD),malondialdehyde(MDA),and total tritium contents during the juvenile stage;and egg production during the adult stage.The t-test was used for intergroup comparison of various detection indicators(equal variance).Results The hatching rates of the three groups of zebrafish in F1 generation were(90.66±0.05)%,(85.63±0.10)%,and(78.06±0.15)%.Compared with the 0 Bq/L tritiated water exposure group,no statistically significant difference was found in the hatching rate of F1 zebrafish between the 1×10^(2) Bq/L and 1×10^(5) Bq/L tritiated water exposure groups(t=0.785,1.370;P=0.462,0.220).The number of autonomous movement of the three groups of zebrafish in F1 generation at 24 h after fertilization was(12.93±2.70),(11.30±0.78),and(10.50±0.80)times/min.Compared with the 0 Bq/L tritiated water exposure group,we observed no statistically significant difference in the number of autonomous movements of F1 generation zebrafish at 24 h after fertilization in the 1×10^(2) Bq/L and 1×10^(5) Bq/L tritiated water exposure groups(t=1.008,1.499;P=0.370,0.208).The number of autonomous movement of the three groups of zebrafish at 36 h after fertilization was(3.63±1.43),(4.50±1.15),and(5.40±3.55)times/min.Compared with the 0 Bq/L tritiated water ex

关 键 词：氚 斑马鱼 F1代 生物效应 

分 类 号：R146[医药卫生—公共卫生与预防医学]