miR-181a-5p对坐骨神经损伤后神经细胞凋亡的影响  

Effect of miR-181a--5p on apoptosis of nerve cells after sciatic nerve injury

作　　者：车敏[1] 王岩峰[2] 李良满[2] 姚阳[1] 张辉[1] CHE Min;WANG Yan-feng;LI Liang-man;YAO Yang;ZHANG Hui(Department of Hand Surgery,Central Hospital Affiliated to Shenyang Medical College,Shenyang 110024;Department of Orthopedic Surgery,The First Affiliated Hospital of China Medical University,Shenyang 110001,China)

机构地区：[1]沈阳医学院附属中心医院手外科,辽宁沈阳110024 [2]中国医科大学附属第一医院骨科,辽宁沈阳110001

出　　处：《解剖科学进展》2023年第5期513-516,520,共5页Progress of Anatomical Sciences

基　　金：国家自然基金(8197080581);辽宁省自然科学基金(2020-MS-146);辽宁省教育厅高校基金(LJKMZ20221781);辽宁省教育厅科学研究面上项目(LJKZ0745)。

摘　　要：目的探究miR-181a-5p对坐骨神经损伤后神经细胞再生及凋亡的影响。方法建立坐骨神经损伤大鼠模型,通过FISH和qRT-PCR检测坐骨神经miR-181a-5p表达。鞘内注射antagomiR-181a-5p,通过qRT-PCR检测坐骨神经miR-181a-5p表达,通过HE染色检测坐骨神经病理学变化,通过TUNEL检测坐骨神经细胞凋亡。结果miR-181a-5p在坐骨神经损伤大鼠坐骨神经近侧断端表达升高。鞘内注射antagomiR-181a-5p降低坐骨神经损伤大鼠坐骨神经miR-181a-5p表达,改善大鼠坐骨神经损伤,降低坐骨神经细胞凋亡水平。结论miR-181a-5p在损伤的坐骨神经中高表达,抑制miR-181a-5p表达降低坐骨神经细胞凋亡水平。Objective To explore the effect of miR-18la-5p on nerve cell regeneration and apoptosis after sciatic nerve injury.Methods A rat model of sciatic nerve injury was established,and the expression of miR-18la-5p in sciatic nerve was detected by FISH and qRT-PCR.antagomiR-181a-5p was injected intracurally,and the expression of miR-181a-5p in sciatic nerve was detected by qRT-PCR.The pathological changes of sciatic neuropathology were detected by HE staining.The apoptosis of sciatic nerve cells was detected by TUNEL.Results miR-18la-5p was increased in the proximal sciatic nerve in rats with sciatic nerve injury.Intracural injection of antagomiR-181a-5p decreased the expression of miR-18la-5p in the sciatic nerve of rats with sciatic nerve injury,improved the sciatic nerve injury and reduced the apoptosis level of sciatic nerve cells.Conclusion miR-18la-5p was highly expressed in injured sciatic nerve,and inhibition of miR-181a-5p reduced the apoptosisof sciatic nerve cells.

关 键 词：miR-181a-5p 坐骨神经损伤 再生 凋亡 

分 类 号：R651.3[医药卫生—外科学]