胶球藻多糖对衰老小鼠前列腺增生的影响及可能机制  

Effect and possible mechanism of coccomyxa gloeobotry diformis polysaccharide on prostate hyperplasia in aging mice

作　　者：王慧 孙鲁宁[1] WANG Hui;SUN Lu-ning(Department of Pathophysiology,College of Basic Medical Science,China Medical University,Shenyang 110122,China)

机构地区：[1]中国医科大学基础医学院病理生理学教研室,辽宁沈阳110122

出　　处：《解剖科学进展》2023年第5期545-547,551,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金(81800763)。

摘　　要：目的探讨胶球藻多糖对衰老小鼠前列腺增生的影响及可能机制。方法选取30只雄性C57BL/6J小鼠,建立自然衰老小鼠模型,随机分为对照组和胶球藻多糖治疗组。HE染色观察各组小鼠前列腺组织学变化;Western blot检测前列腺组织增殖相关蛋白PCNA,CyclinD1和炎症相关蛋白HO-1,COX-2,SOD1的表达水平;免疫组织化学方法检测前列腺组织Ki67的表达情况。结果胶球藻多糖治疗能够显著抑制前列腺组织学增生,减少前列腺组织PCNA、CyclinD1、Ki67的表达;减少COX-2的表达,并上调HO-1、SOD1蛋白水平。结论胶球藻多糖通过减轻前列腺炎症反应抑制衰老小鼠前列腺增生。Objective To investigate the effect of coccomyxa gloeobotry diformis polysaccharide on prostate hyperplasia in aging mice and its possible mechanism.Methods Thirty male C57BL/6J mice were selected to establish a natural aging mouse model and were randomly divided into the control group and coccomyxa gloeobotry diformis polysaccharide treatment group.H&E staining was used to observe the prostate histological changes.The expressions of proliferation-related proteins PCNA,CyclinD1 and inflammation-related proteins HO-1,COX-2,SODI were detected by Western blot.The expression of Ki67 was detected by immunohistochemistry.Results Coccomyxa gloeobotry diformis polysaccharide can significantly inhibit the prostate histological hyperplasia,and it can reduce the expression of PCNA,CyclinD1 and Ki67.In addition,coccomyxa gloeobotry diformis polysaccharide downregulated the expression of COX-2 and upregulated the expressions of HO-1 and SOD1.Conclusion Coccomyxa gloeobotry diformis polysaccharide can significantly inhibit prostate hyperplasia in aging mice by reducing prostate inflammation.

关 键 词：胶球藻多糖 前列腺增生 炎症 药物治疗 细胞增殖 

分 类 号：R697.3[医药卫生—泌尿科学]