大孔树脂分离纯化蜘蛛香总酚酸及其生物活性研究  

Separation and Purification of Total Phenolic Acid From Valeriana Jatamansi Jones With Macroporous Resins and Evaluation on Its Biological Activities

作　　者：程盛勇 胡光线 刘兴赋 沈祥春 罗喜荣 杨军[4] CHENG Sheng-yong;HU Guang-xian;LIU Xing-fu;SHEN Xiang-chun;LUO Xi-rong;YANG Jun(Department of Basic Medicine,Guizhou Nursing Vocational College,Guiyang Guizhou 550025,China;Guizhou Zhongguan Biotechnology Co.,Ltd.,Guiyang Guizhou 550081,China;School of Pharmaceutical Sciences,Guizhou Medical University,Guiyang Guizhou 550025,China;Institute of Geochemistry,Chinese Academy of Sciences,Guiyang Guizhou 550081,China)

机构地区：[1]贵州护理职业技术学院基础医学部,贵州贵阳550025 [2]贵州中观生物技术有限公司,贵州贵阳550081 [3]贵州医科大学药学院,贵州贵阳550025 [4]中国科学院地球化学研究所,贵州贵阳550081

出　　处：《当代化工》2023年第12期2876-2882,共7页Contemporary Chemical Industry

基　　金：贵州省科技创新基地(黔科合中引地[2023]003);贵州护理职业技术学院硕士基金课题(项目编号:gzhlyj2022-06)。

摘　　要：优化AB-8大孔树脂富集纯化蜘蛛香中总酚酸的工艺条件,并测定其抗氧化、抗乙酰胆碱酯酶的生物活性。以吸附率、解析率、总酚酸含量为评价指标,优化AB-8大孔树脂对蜘蛛香总酚酸的纯化工艺条件,并集成溶剂萃取法制备质量分数≥50%蜘蛛香总酚酸,采用清除DPPH·和PTIO·的方法评价蜘蛛香总酚酸的抗氧化活性,采用改良版的Ellman比色法测定蜘蛛香总酚酸抗乙酰胆碱酯酶活性。结果表明,最佳工艺条件为:上样质量浓度为2.66 mg·L^(-1)、上样液p H=4.6、上样流速1 BV·h^(-1)、上样体积2 BV、水洗用量5 BV、除杂剂20%乙醇4 BV、洗脱剂50%乙醇4 BV;粗提液先用有机溶剂萃取再经大孔树脂分离纯化得57.77%蜘蛛香总酚酸;纯化后样品对DPPH·、PTIO·有较好的清除能力和有较好的抗乙酰胆碱酯酶活性,IC50值分别为0.04 mg·m L^(-1)、2.00 mg·m L^(-1)、5.40 mg·m L^(-1)。优化所得工艺稳定可行,纯化的蜘蛛香总酚酸具有较好的抗氧化和抗乙酰胆碱酯酶活性,为其研究开发提供一定的参考依据。The process conditions were optimized for the enrichment and purification of total phenolic acid in valeriana jatamansi jones by AB-8 macroporous resin,and its antioxidant and anti-acetylcholinesterase biological activities were determined.Using adsorption rate,resolution rate,and total phenolic acid content as evaluation indicators,the purification process conditions of AB-8 macroporous resin for total phenolic acids of valeriana jatamansi jones incense were optimized,and the solvent extraction method was integrated to prepare the total phenolic acids of Valeriana jatamansi Jones incense with a mass fraction of≥50%,the antioxidant activity of valeriana jatamansi jones total phenolic acid was evaluated by clearing DPPH·and PTIO·,and the anti-acetylcholinesterase activity of valeriana jatamansi jones total phenolic acid was determined by the improved Ellman colorimetric method.The results showed that the optimal process conditions were as follows:sample mass concentration 2.66 mg·mL^(-1),sample solution pH=4.6,sample flow rate 1 BV·h^(-1),sample volume 2 BV,water washing amount 5 BV,impurity remover 20%ethanol of 4 BV,eluent 50%ethanol of 4 BV.The crude extract was first extracted with organic solvents,and then separated and purified by macroporous resin to obtain 57.77%total phenolic acid from valeriana jatamansi jones.After being purified,the samples exhibited significant scavenging ability and anti-acetylcholinesterase activity against DPPH·and PTIO·,with IC50 values of 0.04 mg·mL^(-1),2.00 mg·mL^(-1),and 5.40 mg·mL^(-1),respectively.The optimized process demonstrates stability and feasibility,while the purified arachnophenolic acid exhibits commendable antioxidant and anti-acetylcholinesterase activities.These findings provide valuable insights for its further research and development.

关 键 词：蜘蛛香 总酚酸 AB-8大孔树脂 分离纯化 抗氧化 抗乙酰胆碱酯酶 

分 类 号：TQ028[化学工程]