UPLC-QAMS法同时测定珍珠胃安丸中甘草苷等10个成分的含量  

Simultaneous Quantitative Determination of Ten Components Iiquiritin in Pearl Wei'an Pills by an UPLC-QAMS Method

作　　者：刘艳云 梁少东 蒋居琼 张铨 伍国怡 LIU Yanyun;LIANG Shaodong;JIANG Juqiong;ZHANG Quan;WU Guoyi(Beihai Institute for Food and Drug Control,Beihai,Guangxi 536000,China)

机构地区：[1]北海市食品药品检验所,广西北海536000

出　　处：《药学与临床研究》2023年第6期502-507,共6页Pharmaceutical and Clinical Research

基　　金：北海科技计划项目[北科字(2020)165号]。

摘　　要：目的:建立超高压液相色谱结合一测多评(UPLC-QAMS)法同时测定珍珠胃安丸中甘草苷、芸香柚皮苷、橙皮苷、芹糖异甘草苷、异甘草苷、甘草素、丹皮酚、异甘草素、甘草酸铵、川陈皮素的含量。方法:采用超高压液相色谱法,使用Athena UHPLC C_(18) Column,120A(2.1 mm×150 mm,1.8μm)色谱柱,流动相为乙腈-0.05%磷酸溶液,梯度洗脱,流速为0.3 mL·min^(-1),柱温35℃,检测波长为237 nm。以丹皮酚为参照物,确定其与其他9个成分的相对校正因子,QAMS法与外标法分别测定珍珠胃安丸中10个成分的含量。结果:在0.28~333.54μg·mL-1线性范围内,10个成分呈良好的线性关系(r^(2)> 0.999 9),平均加样回收率为98.55%~101.77%(n=6),RSD 1.33%~2.97%(n=6)。丹皮酚与甘草苷、芸香柚皮苷、橙皮苷、芹糖异甘草苷、异甘草苷、甘草素、异甘草素、甘草酸铵、川陈皮素的相对校正因子分别为0.276、0.699、0.439、0.583、0.423、0.172、0.320、1.013、0.541,外标法与QAMS法所测得10个成分的含量无明显差异。结论:本研究建立的QAMS法准确性高,可为珍珠胃安丸质量控制标准的提高提供依据。Objective:To establish an UPLC-QAMS method for the simultaneous determination of liquiritin,narirutin,hesperidin,isoliquiritin apioside,isoliquiritin,liquiritigenin,paeonol,isoliquiritigenin,ammonium glycyrrhizinate and nobiletin in Pearl Wei’an pills.Methods:Ultrahigh pressure liquid chromatography was performed on an Athena UHPLC C_(18) Column on 120A(2.1 mm×150 mm,1.8μm)with mobile phase consisting of acetonitrile-0.05%phosphoric acid solution for gradient elution at a flow rate of 0.3 mL·min^(-1).The column temperature was 35℃ and the detection wavelength was 237 nm.Using paeonol as reference material,the relative correction factors of paeonol with liquiritin,narirutin,hesperidin,isoliquiritin apioside,isoliquiritin,liquiritigenin,isoliquiritigenin,ammonium glycyrrhizate and nobiletin were determined.QAMS method and external standard method were used to determine the contents of the 10 components in Pearl Wei’an pills.Results:In the range of 0.28-333.54μg·mL^(-1),the 10 components showed a good linear relationship(r^(2)>0.9999).The average recoveries were 98.55%-101.77%(n=6),the RSD were 1.33%-2.97%(n=6).The relative correction factors of paeonol and liquiritin,narirutin,hesperidin,isoliquiritin apioside,isoliquiritin,liquiritigenin,isoliquiritigenin,ammonium glycyrrhizinate and nobiletin were 0.276,0.699,0.439,0.583,0.423,0.172,0.320,1.013 and 0.541,respectively.There was no significant difference between the external standard method and the QAMS method in measuring the 10 components.Conclusion:The QAMS method established in this study has high accuracy,which can provide a basis for improving the quality control standard of Pearl Wei’an pills.

关 键 词：珍珠胃安丸 超高压液相法 一测多评 含量测定 

分 类 号：R927.2[医药卫生—药学]