高效液相色谱-荧光法测定饲料中维生素B_(1)和维生素B_(2)  

Determination of vitamin B_(1) and vitamin B_(2) in feed by HPLC with fluorescence

作　　者：唐雯 黄媛 TANG Wen;HUANG Yuan(Fujian Inspection and Research Institute for Product Quality,Fuzhou,Fujian Province 350015,China)

机构地区：[1]福建省产品质量检验研究院,福建福州350015

出　　处：《中国饲料》2024年第3期113-117,共5页China Feed

摘　　要：为建立以高效液相色谱-荧光法测定饲料中维生素B_(1)和维生素B_(2)的分析方法,实验通过高温高压法对饲料进行前处理,而后衍生,以乙酸钠-甲醇为流动相,分别在不同波长下对维生素B_(1)和B_(2)进行测定。结果显示:维生素B_(1)和维生素B_(2)在0.03~5.00μg/mL线性范围内,相关系数R均大于0.9999;加标回收率为92.7%~98.1%;维生素B_(1)和维生素B_(2)的检出限分别为0.04、0.06 mg/kg,定量限分别为0.15、0.18 mg/kg。本方法对饲料中维生素B_(1)和维生素B_(2)的测定,具有便捷、准确的优势。To establish an analytical method for the determination of vitamin B_(1) and vitamin B_(2) in feed by high performance liquid chromatography-fluorescence,the feed was pretreated by high temperature and high pressure method,and the extract was derivatized.Vitamin B_(1) and vitamin B_(2) were determined at different wavelengths with sodium acetate-methanol as mobile phase.The results showed that the liner range were 0.03~5.00μg/mL.The correlation coefficients were greater than 0.9999.The recoveries were 92.7%~98.1%.The limit of detection respective were 0.04 mg/kg and 0.06 mg/kg of vitamin B_(1) ang vitamin B_(2).The limit of quantitation respective were 0.15 mg/kg and 0.18 mg/kg of vitamin B_(1) ang vitamin B_(2).This method was convenient and accurate for the determination of vitamin B_(1) and B_(2) in feed.

关 键 词：高效液相色谱-荧光法 饲料 维生素B_(1)和维生素B_(2) 

分 类 号：S816.17[农业科学—饲料科学]