种苗期川党参根际土壤细菌群落变化研究  

Study on the Changes of Bacterial Community in Rhizosphere Soil of Codonopsis Tangshen Oliv.at Seedling Stage

作　　者：罗敏[1] 邓才富[1] 朱晓富 徐广[1] 罗舜[1] Luo Min;Deng Caifu;Zhu Xiaofu;Xu Guang;Luo Shun(Chongqing Institute of Medicinal Plant Cultivation,Chongqing 408435,China)

机构地区：[1]重庆市药物种植研究所,重庆408435

出　　处：《亚太传统医药》2024年第1期51-55,共5页Asia-Pacific Traditional Medicine

基　　金：重庆市自然科学基金面上项目(cstc2020jcyj-msxmX0470);重庆市科研机构绩效激励引导专项(cstc2021-jxjl007;cstc2018jxjl-jbky130019);重庆市科技局基本科研业务费专项项目(23JK78-2312)。

摘　　要：目的:研究川党参种苗期根际土壤细菌群落的结构特征和多样性变化,掌握种苗期土壤微生物群落的演替规律,为川党参连作障碍的防治提供理论依据。方法:采集1年生(Op1)和2年生(Op2)的川党参根际土壤,采用Illumina MiSeq高通量测序技术进行细菌16S rRNA序列V3-V4区的高通量测序,对种苗期根际土壤细菌群落变化进行分析。结果:通过MiSeq测序共得到882369条优化序列,其中1年生注释到9926个OTU,2年生注释到7560个OTU。种苗期随生长年限的增加,川党参根际土壤速效养分、细菌群落丰富度和多样性均呈增加趋势。在细菌门分类水平上,1年生有5个优势菌群(相对丰度均大于5%),2年生有7个优势菌群,共有优势菌群为变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)、放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes);在属分类水平上排名前五菌群中只有全噬菌属(Holophaga)与念珠菌属(Candidatus Udaeobacter)为共有优势菌群,且1年生有11个独有菌群。PCoA分析与UPGMA聚类分析表明1年生与2年生川党参根际土壤细菌群落具有显著差异,随生长年限延长根际土壤细菌群落结构演变明显。结论:川党参种苗期根际土壤速效养分、土壤细菌群落多样性呈递增变化,细菌群落结构相似性呈递减变化。研究为揭示川党参种植和连作障碍形成的原因提供了理论依据。Objective:To study the structural characteristics and diversity of bacterial community in rhizosphere soil of Codonopsis tangshen at seedling stage,and to grasp the succession law of soil microbial community at seedling stage,so as to provide theoretical basis for the prevention and control of continuous cropping obstacles of C.tangshen.Methods:The rhizosphere soil of 1-year-old(Op1)and 2-year-old(Op2)Codonopsis tangshen was collected,the high-throughput sequencing of V3-V4 region of bacterial 16 S rRNA sequence was carried out by Illumina MiSeq high-throughput sequencing technology to analyze the changes of bacterial community in rhizosphere soil at seedling stage.Results:A total of 882369 optimized sequences were obtained by MiSeq sequencing,of which 9926 OTUs were annotated in 1-year-old and 7560 OTUs were annotated in 2-year-old.With the increase of growth years,the available nutrients,bacterial community richness and diversity in the rhizosphere soil of C.tangshen increased.At the level of bacterial phylum classification,there were 5 dominant bacteria in 1-year-old seedlings(relative abundance was greater than 5%)and 7 dominant bacteria in 2-year-old seedlings.The common dominant bacteria were Proteobacteria,Acidobacteria,Actinobacteria and Bacteroidetes.Among the top five genera,only Holophaga and Candidatus Udaeobacter were the common dominant genera,and there were 11 unique genera in one year.PCoA and UPGMA cluster analysis showed that the bacterial community in the rhizosphere soil of one-year-old and two-year-old C.tangshen was significantly different,and the bacterial community structure in the rhizosphere soil evolved significantly with the extension of growth years.Conclusion:The rhizosphere soil available nutrients and soil bacterial community diversity of C.tangshen at seedling stage showed an increasing change,and the similarity of bacterial community structure showed a decreasing change,which provided a theoretical basis for the cultivation of C.tangshen and the formation of continuous cropping o

关 键 词：川党参 根际土壤 细菌群落 种苗期 

分 类 号：R282.2[医药卫生—中药学] S567[医药卫生—中医学] S154.3[农业科学—中草药栽培]