逆境胁迫对拟南芥细胞质、叶绿体和细胞外ATP水平的影响  

Different effects of adversity stresses on cytosolic,chloroplastic,and extracellular ATP levels in Arabidopsis thaliana

作　　者：李丽[1] 达晓伟 张悦婧 杨红红 石珍珍 贾凌云[1] 冯汉青[1] LI Li;DA Xiao-wei;ZHANG Yue-jing;YANG Hong-hong;SHI Zhen-zhen;JIA Ling-yun;FENG Han-qing(College of Life Science,Northwest Normal University,Lanzhou 730070,China)

机构地区：[1]西北师范大学生命科学学院,兰州730070

出　　处：《兰州大学学报（自然科学版）》2023年第6期846-852,共7页Journal of Lanzhou University(Natural Sciences)

基　　金：国家自然科学基金项目(31870246,31560070);甘肃省重点研发计划项目(22YF7NA119);甘肃省高等学校产业支撑计划项目(2023CYZC-20);甘肃省科技重大专项(22ZD1NA001);西北师范大学新农村发展研究院开放基金;西北师范大学研究生科研项目(2021KYZZ01037);西北师范大学优秀博士论文培养项目;甘肃省科技计划(优秀博士)项目(23JRRA697,23JRRA707)。

摘　　要：利用荧光共振能量转移的ATeam蛋白作为ATP生物传感器,建立检测拟南芥细胞质、叶绿体和细胞外ATP的检测体系,研究植物在不同非生物胁迫(NaCl、 PEG 6000和低温)下细胞质、叶绿体和细胞外ATP水平的变化.结果表明,在较高强度的胁迫(200 mmol/L NaCl、 10%~20%w(PEG6000)和0~4℃)下,细胞质ATP水平下降.在各水平PEG 6000胁迫下,叶绿体中ATP水平保持稳定;10℃低温处理导致叶绿体中ATP水平升高,200 mmol/L NaCl处理导致叶绿体ATP水平下降,其他强度的NaCl以及低温处理未显著改变叶绿体ATP水平.在中等强度的NaCl和PEG 6000胁迫(100 mmol/L NaCl和5%~10%w(PEG 6000))下,细胞外ATP水平升高;4~10℃低温胁迫处理导致细胞外ATP水平下降.A fluorescence resonance energy transfer ATP biosensor based on ATeam protein was established to detect the cytosolic,chloroplastic and extracellular ATP levels in Arabidopsis thaliana.By means of these A.thaliana lines,the changes of cytosolic,chloroplastic and extracellular ATP levels in A.thaliana were investigated under different abiotic stresses(NaCl,PEG 6000,and low temperature).It was found that the level of cytosolic ATP decreased under a higher intensity of the abiotic stresses(200 mmol/L NaCl,10%-20% PEG 6000,and 0-4 ℃).The level of chloroplastic ATP was stable under different levels of PEG 6000 stress.The level of chloroplastic ATP was increased under 10 ℃,but decreased under 200 mmol/L NaCl.There were not significantly changes inchloroplastic ATP levels under other intensities of NaCl and low temperature stresses.The level of extracellular ATP was increased under a moderate intensity of the NaCl and PEG 6000 stresses(100 mmol/L NaCl and 5%-10% PEG 6000),while it decreased under 4-10 ℃ condition.

关 键 词：拟南芥 ATP水平 盐胁迫 PEG 6000胁迫 低温 荧光共振能量转移 

分 类 号：Q945.79[生物学—植物学]