柴胡疏肝散治疗非酒精性脂肪肝的网络药理学机制  

Molecular mechanism of Chaihu Shugan Powder in the treatment of nonalcoholic fatty liver disease based on network pharmacology

作　　者：苏陈颖 朱尉东 郑琳[3] 江睿轩 郑海音[2] 林凡[2] SU Chenying;ZHU Weidong;ZHENG Lin;JIANG Ruixuan;ZHENG Haiyin;LIN Fan(Fujian University of Traditional Chinese Medicine,FuJian Province,Fuzhou350122,China;College of Integrative Medicine,Fujian University of Traditional Chinese Medicine,FuJian Province,Fuzhou350122,China;Department of Gastroenterology,People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine,FuJian Province,Fuzhou350004,China)

机构地区：[1]福建中医药大学,福建福州350122 [2]福建中医药大学中西医结合学院,福建福州350122 [3]福建中医药大学附属人民医院消化科,福建福州350004

出　　处：《中国当代医药》2024年第2期4-8,共5页China Modern Medicine

基　　金：福建省大学生创新创业训练计划项目(202110393001);福建省自然科学基金资助项目(2018J01325)。

摘　　要：目的基于网络药理学探究柴胡疏肝散治疗非酒精性脂肪肝(NAFLD)的作用机制。方法检索TCMSP数据库获取柴胡疏肝散活性成分及靶标,通过Gene Cards获取NAFLD相关靶标,随后利用Omicshare数据库获取药物及疾病的交集靶标并进行富集分析。借用STRING数据库对交集靶标构建PPI。利用cytoscape软件分析所得网络图,得到柴胡疏肝散治疗NAFLD的关键化合物及关键靶标。结果筛得柴胡疏肝散治疗NAFLD关键化合物4个,关键靶标包括蛋白激酶(AKT1)、白细胞介素-6(IL-6)、肿瘤坏死因子(TNF)、肿瘤蛋白p53(TP53)和血管内皮生长因子A(VEGFA),涉及AGE-RAGE、NAFLD、核因子-κB(NF-κB)等信号通路。结论柴胡疏肝散通过多成分激活AGE-RAGE和NAFLD等信号通路治疗NAFLD,为临床应用提供理论新依据。Objective To explore the mechanism of Chaihu Shugan Powder on nonalcoholic fatty liver disease(NAFLD)based on network pharmacology.Methods The TCMSP database was searched to screen the active components and targets of Chaihu Shugan Powder.The targets of liver related diseases were obtained through GeneCards.Then the common targets of drugs and diseases were obtained and analyzed by Omicshare.STRING database was used to construct PPI for common targets.The related network diagrams of active components and common targets were analyzed by cytoscape software to obtain the key compounds and key targets of Chaihu Shugan Powder in the treatment of NAFLD.Results Four key compounds were identified in the treatment of NAFLD,including RAC-alpha serine/threonine-protein kinase(AKT1),interleukin-6(IL-6),tumor necrosis factor(TNF),cellular tumor antigen p53(TP53),vascular endothelial growth factor A(VEGFA),involving AGE-RAGE,NAFLD,nuclear factor-κB(NF-κB)signaling pathways.Conclusion Chaihu Shugan Powder can treat NAFLD through multi-component group activation of AGE-RAGE and NAFLD and other pathways,and provide a new theoretical basis for clinical application.

关 键 词：非酒精性脂肪肝 柴胡疏肝散 网络药理学 信号通路 

分 类 号：R274.9[医药卫生—中西医结合]