利勃隆促进雄性少精症斑马鱼性行为和性腺发育及精子数量产生  

Spermary promotes sexual behavior,gonadal development and sperm count production in male zebrafish

作　　者：赵成瑞[1] 郑新越 常明亮[1] 康志云 ZHAO Chengrui;ZHENG Xinyue;CHANG Mingliang;KANG Zhiyun(Fenyang College of Shanxi Medical University,Fenyang,Shanxi 032200,China;Beijing No.1 Biochemical Pharmaceutical Co.,Ltd.,Beijing 101400,China)

机构地区：[1]山西医科大学汾阳学院,山西汾阳032200 [2]北京第一生物化学药业有限公司,北京101400

出　　处：《重庆医学》2024年第2期176-179,187,共5页Chongqing medicine

基　　金：山西省吕梁市重点研发项目(2021SHFZ-2-100)。

摘　　要：目的探索利勃隆对性行为、性腺发育及精子数量产生的影响。方法56尾受精后10月龄AB品系雄性斑马鱼分7缸设为正常组,模型组,2个阳性对照组(枸橼酸氯米芬组,五子衍宗丸组),利勃隆低、中、高浓度组,每组8尾。除正常组外,分别水溶给予环磷酰胺建立斑马鱼少精症模型。模型建立第9天,阳性对照组按照枸橼酸氯米芬组0.1μg/mL浓度、五子衍宗丸组6μg/mL浓度分别给予相应药物;利勃隆低、中、高浓度组按200μg/mL、300μg/mL、900μg/mL浓度给予相应药物。模型建立第16天,捞取正常雌性斑马鱼按1∶1置于各组培养缸,观察并记录雄雌鱼追尾频数后捞取雄性斑马鱼,测量并统计体长、体重、精巢重量及精子数量。对用药前、后和各组间差异(one-way ANOVA)进行统计学分析。结果与正常组比较,模型组斑马鱼追尾频数、体长、体重、精巢重量、精子数量均明显减少(P<0.05);与模型组比较,利勃隆低、中、高浓度组斑马鱼追尾频数、体长、体重、精巢重量、精子数量均明显增加(P<0.05)。其中,利勃隆中浓度组斑马鱼性行为活跃度改变最明显,精子数量增加最明显(P<0.01)。结论一定剂量利勃隆能有效促进雄性少精症斑马鱼性行为、性腺发育及精子数量产生。利勃隆可提升男性性功能和治疗少、弱精症方面的功效值得研究。Objective To explore the effects of spermary on sexual behavior,gonadal development and sperm count production.Methods Fifty-six male zebrafishes of 10-month-old AB strain after fertilization were randomly divided into 7 tanks and set up as the normal group,model group,positive control group(clomiphene citrate group,Wuzi Yanzong Pills group),and Spermary low,medium and high concentration groups,8 fishes in each group.Except for the normal group,cyclophosphamide was given in water solution to establish the zebrafish oligospermia model,respectively.On 9 d of modeling,the positive control group was administered at a concentration of 0.1μg/mL in the clomiphene citrate group and 6μg/mL in the Wuzi Yanzong Pill group;the spermary low,medium and high concentration groups were administered at 200,300,900μg/mL,respectively.On 16 d of modeling,the normal female zebrafishes were placed in the culture tank of each group in a 1∶1 ratio,and the frequency of tail-chasing was observed and recorded in male and female fishes.The body length,weight,te stis weight and sperm count were measured and the statistical analysis was performed by comparing the differences among the groups before and after drug administration(one-way ANOVA).Results Compared with the normal group,the fre quency of tail-chasing of zebrafishes,body length,body weight,testis weight and sperm count in the model group were significantly decreased(P<0.05).Compared with the model group,the frequency of tail-chasing of zebrafishes,body length,body weight,testis weight and sperm count in the spermary low,medium and high concentration groups all were significantly increased(P<0.05),in which the vitality change of sexual behavior in the spermary medium concentration group was most obvious,and the increase of sperms count was most obvious.Conclusion A certain dose of spermary could effectively promote the sexual behavior,gonadal development and sperm count production in male zebrafish with oligospermia.The efficacy of spermary in enhancing the male sexual function a

关 键 词：利勃隆 斑马鱼 少精症 弱精症 睾酮 

分 类 号：R698.2[医药卫生—泌尿科学]