基于生物3D打印技术的水凝胶器官芯片及其灌注平台  

Hydrogel Organ Chip Based on 3D Bioprinting Technology and Its Perfusion Platform

作　　者：宋曙瞳 李松 张黎明[2,3] 王雨婕[2,3,4] Song Shutong;Li Song;Zhang Liming;Wang Yujie(School of Mechanical Engineering,Shenyang Ligong University,Shenyang 110159,China;State Key Laboratory of Robotics,Shenyang Institute of Automation,Chinese Academy of Sciences,Shenyang 110016,China;Institutes for Robotics and Intelligent Manufacturing,Chinese Academy of Sciences,Shenyang 110169,China;College of Information Engineering,Shenyang University of Chemical Technology,Shenyang 110142,China)

机构地区：[1]沈阳理工大学机械工程学院,沈阳110159 [2]中国科学院沈阳自动化研究所机器人学国家重点实验室,沈阳110016 [3]中国科学院机器人与智能制造创新研究院,沈阳110169 [4]沈阳化工大学信息工程学院,沈阳110142

出　　处：《微纳电子技术》2024年第1期87-94,共8页Micronanoelectronic Technology

基　　金：国家重点研发计划项目(2020YFB1313100);国家自然科学基金面上项目(51875557)。

摘　　要：针对水凝胶组织支架细胞球培养过程中存在着缺乏相关设备和实验平台的问题,采用生物3D打印技术和动态灌注培养的方法,设计制作了一种双通道水凝胶器官芯片,并搭建了一套灌注培养平台。采用聚苯乙烯荧光微球模拟细胞球的灌注过程,通过有限元仿真分析了不同流速下(0.849、2.547、4.244 mm/s)微球灌注时,通道不同位置流速和剪切应力的变化。设计了聚苯乙烯荧光微球动态灌注实验,基于仿真结果分析了流速对微球流动状态与贴附的影响。实验结果表明:灌注液在通道截面中心位置的流速最大,在通道周围趋近于静止,有利于微球沉降。灌注液流速为2.547 mm/s时,微球受到的最大剪切应力约为0.17 Pa,可以大量堆积在通道分支内。该研究可以为胰岛等细胞球在水凝胶器官芯片内的灌注和培养提供技术参考。To resolve the problem of the lack of relative equipment and experimental platform in the cell sphere culture process of hydrogel tissue scaffold,a dual-channel hydrogel organ chip was designed and fabricated by 3D bioprinting technology and dynamic perfusion culture method,and a set of perfusion culture platform was constructed.Polystyrene fluorescent microspheres were used to simulate the perfusion process of cell spheres.The changes of flow velocities and shear stresses at different positions of the channel were analyzed by finite element simulation at different flow velocities(0.849,2.547 and 4.244 mm/s).The dynamic perfusion experiment of the polystyrene fluorescent microspheres was designed.Based on the simulation results,the influences of flow velocity on the flow state and attachment of the microspheres were analyzed.The experimental results show that the flow velocity of the perfusion fluid at the center of the channel section is the largest,and the perfusion fluid tends to be stationary around the channel,which is beneficial to the sedimentation of the microspheres.When the perfusion fluid flow velocity is 2.547 mm/s,the maximum shear stress of the microspheres is 0.17 Pa,which can be accumulated in channel branches in large numbers.The study can provide technical reference for perfusion and culture of islets and other cell spheres in hydrogel organ chips.

关 键 词：生物3D打印 器官芯片 水凝胶 动态灌注 荧光微球 

分 类 号：TH703[机械工程—仪器科学与技术]