基于DLP 3D打印的高给药效率微针制备工艺  

Preparation Process of Microneedles with High Drug Delivery Efficiency Based on DLP 3D Printing

作　　者：常兆敏 刘瑛[1] 亓剑[1] 郑淑贤[2] Chang Zhaomin;Liu Ying;Qi Jian;Zheng Shuxian(Tianjin Key Laboratory of High Speed Cutting and Precision Machining,Tianjin Engineering Research Center for High-End Intelligent CNC Machine Tools,School of Mechanical Engineering,Tianjin University of Technology and Education,Tianjin 300350,China;Tianjin Key Laboratory of Equipment Design and Manufacturing Technology,Tianjin University,Tianjin 300354,China)

机构地区：[1]天津职业技术师范大学机械工程学院、天津市高端智能数控机床工程研究中心、天津市高速切削与精密加工重点实验室,天津300350 [2]天津大学装备设计与制造技术天津市重点实验室,天津300354

出　　处：《微纳电子技术》2024年第1期144-152,共9页Micronanoelectronic Technology

基　　金：国家自然科学基金项目(52175275);天津市研究生科研创新项目(2022SKYZ130);天津市教委科研计划项目(2021KJ032);天津职业技术师范大学预研项目(KYQD202210);2023年天津市技术创新引导专项企业科技特派员项目。

摘　　要：载药微针可以实现一次性快速药物递送,无需二次药物涂敷与输送过程,但其不佳的载药量与刺入率造成了给药效率差的问题。研发了一种基于数字光处理(DLP)3D打印技术的高给药效率微针(HDMN)制备工艺。通过在载药微针上构建锯齿状针体结构与阶梯状载药结构来提高载药微针的刺入率与载药量,进而提高给药效率。实验结果表明使用环氧丙烯酸酯(EA)打印微针时,当曝光时间为700 ms、打印层厚为30μm时,打印出的微针实际尺寸与理论尺寸平均误差最小为9.2%。利用旋涂法在微针表面交联聚乙二醇二丙烯酸酯(PEGDA)以改善微针生物相容性。显微镜观察发现,当交联层数为1层时,PEGDA均匀地覆盖在微针表面。石蜡膜穿刺实验结果表明,HDMN穿透了2层石蜡膜,而相同实验条件下的传统载药微针仅穿透了1层。药物释放实验结果表明,HDMN相对于传统载药微针在猪皮内留下的模拟药物浓度提升了61%。该研究为后续通过改善微针结构来优化微针性能研究提供了有益借鉴。Drug-loading microneedles can achieve one-time rapid drug delivery without secondary drug coating and delivery processes,but their poor drug loading capacity and penetration rate cause poor drug delivery efficiency.The fabrication process of high drug delivery efficiency microneedles(HDMNs)based on digital light processing(DLP)3D printing technology was developed.By constructing a serrated needle structure and a ladder-shaped drug-loading structure on the drug-loading microneedles,the penetration rate and drug loading capacity of the drugloading microneedles were improved,and then the drug delivery efficiency was improved.The experimental results show that when printing microneedles using epoxy acrylate(EA),the average error between the actual size and the theoretical size of the printed microneedles is at least 9.2%under the conditions of an exposure time of 700 ms and a printing layer thickness of 30μm.The spin-coating method was used to crosslink polyethylene glycol diacrylate(PEGDA)on the surface of the microneedles to improve the biocompatibility of the microneedles.Microscopic observation result shows that when the number of cross-linked layer is 1,PEGDA is uniformly covered on the surface of the microneedles.The experimental results of paraffin film puncture show that HDMNs penetrate two layers of paraffin films,while the traditional drugloaded microneedles only penetrate one layer under the same experimental conditions.The drug release experimental results show that compared with the traditional drug-loaded microneedles,the simulated drug concentration of HDMNs in pig skin is increased by 61%.The study provides a useful reference for further research on optimizing the performance of microneedles by improving the structure of microneedles.

关 键 词：3D打印 微针结构 数字光处理(DLP) 载药量 刺入率 高给药效率微针(HDMN) 

分 类 号：TH162[机械工程—机械制造及自动化]