麦冬多糖对破骨细胞分化的影响研究  

Effect of Ophiopogon japonicus polysaccharides on osteoclast differentiation

作　　者：易倩 张合富 李官翔 樊丽莎 邓加加 张伶燕 张健 YI Qian;ZHANG Hefu;LI Guanxiang;FAN Lisha;DENG Jiajia;ZHANG Lingyan;ZHANG Jian(Shool of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou 520025,China;Institute for Laboratory Animal Research,Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou 520025,China)

机构地区：[1]贵州中医药大学药学院,贵州贵阳520025 [2]贵州中医药大学实验动物研究所,贵州贵阳520025

出　　处：《现代医药卫生》2024年第2期190-195,共6页Journal of Modern Medicine & Health

基　　金：贵州中医药大学大学生创新创业训练计划项目[贵中医大创合字(2021)79号]。

摘　　要：目的探讨麦冬多糖(OPS)对破骨细胞分化的影响。方法从C57BL/6j小鼠股骨中提取骨髓单核巨噬细胞(BMMs),在细胞培养液中分别添加2.5、10.0和40.0μg/mL OPS(A、B、C组),对照组未添加OPS。检测OPS对BMMs细胞活性、破骨细胞骨吸收能力及抗酒石酸酸性磷酸酶(TRAP)活性的影响,同时分析OPS对碳酸酐酶2(Car2)、组织蛋白酶K(CTSK)、基质金属蛋白酶9(MMP-9)、TRAP、液泡型H^(+)-ATP酶(V-ATPase)mRNA表达的影响。结果A、B、C组48、96 h时吸光度值比较,差异无统计学意义(P>0.05)。与对照组(1.00±0.15)比较,B组成熟破骨细胞数目降低至(0.61±0.06)(P<0.05),而C组成熟破骨细胞数目为(0.31±0.03)(P<0.05)。与对照组相比,B、C组5 d时破骨细胞TRAP活性显著降低(P<0.05)。A、B、C组7 d时破骨细胞TRAP活性显著低于对照组(P<0.05)。A、B、C组骨吸收面积仅为对照组的71%、40%和6%(P<0.05)。与对照组比较,B、C组Car2、CTSK、MMP-9、TRAP、V-ATPase mRNA表达水平显著降低(P<0.01)。结论OPS可显著抑制破骨细胞分化功能,是一种潜在的用于骨质疏松治疗的抗骨吸收药物。Objective To explore the effect of Ophiopogon japonicus polysaccharides(OPS)on osteoclast differentiation.Methods Bone marrow mononuclear macrophages(BMMs)were extracted from the femur of C57BL/6j mice,and 2.5,10.0 and 40.0μg/mL OPS were added to the cell culture medium,respectively(groups A,B and C),while no OPS was added to the control group.The effects of OPS on BMMs cell activity,osteoclast bone resorption capacity and tartrate-resistant acid phosphatase(TRAP)activity were detected.Meanwhile,the effects of OPS on the mRNA expression of carbonic anhydrase 2(Car2),cathepsin K(CTSK),matrix metalloproteinase 9(MMP-9),TRAP and vacuolar H^(+)-ATPase(V-ATPase)were analyzed.Results There was no significant difference in absorbance of group A,B and C at 48 and 96 h(P>0.05).Compared with the control group(1.00±0.15),the number of mature osteoclasts in group B was reduced to(0.61±0.06)(P<0.05),and that in group C was(0.31±0.03)(P<0.05).Compared with the control group,TRAP activity of osteoclasts in group B and C at 5th day was significantly decreased(P<0.05).The TRAP activity of osteoclasts in group A,B and C at 7th day was significantly lower than that in control group(P<0.05).The bone resorption area of group A,B and C was only 71%,40%and 6%of that of the control group(P<0.05).Compared with the control group,the mRNA expression levels of Car2,CTSK,MMP-9,TRAP,and V-ATPase in group B and C were significantly decreased(P<0.01).Conclusion OPS can significantly inhibit osteoclast differentiation and bone resorption,which is a potential anti-resorption drug for osteoporosis treatment.

关 键 词：破骨细胞 麦冬多糖 分化 骨吸收 骨质疏松 

分 类 号：R982[医药卫生—药品]