Box-Behnken响应面法优选紫斑牡丹中丹皮酚和芍药苷提取工艺  

Optimization of Extraction Process of Paeonol and Paeoniflorin from Paeonia Rockii by the Box-Behnken Response Surface Methodology

作　　者：周坤 付永莉[1] 罗婷[1] 罗星 吴春芳[1] ZHOU Kun;FU Yongli;LUO Ting;LUO Xing;WU Chunfang(Wuhan Mental Health Center·Wuhan Hospital for Psychotherapy,Wuhan,Hubei,China 430012)

机构地区：[1]湖北省武汉市精神卫生中心·湖北省武汉市心理医院,湖北武汉430012

出　　处：《中国药业》2024年第3期68-73,共6页China Pharmaceuticals

基　　金：湖北省卫生健康委员会联合基金项目[WJ2019H426];湖北省武汉市医学科研项目[WX19B03]。

摘　　要：目的优选紫斑牡丹中丹皮酚和芍药苷的提取工艺。方法在单因素试验基础上,以液料比、提取时间和乙醇体积分数为影响因素,以丹皮酚和芍药苷提取率的总权重综合评分为评价指标,采用Box-Behnken响应面法优选最佳提取工艺,并进行验证试验。结果最佳提取工艺为液料比28倍,提取时间150 min,乙醇体积分数52%。此工艺条件下,丹皮酚提取率为0.49%(RSD=1.33%,n=3),芍药苷提取率为5.38%(RSD=1.07%,n=3)。结论该提取工艺稳定、可行,可用于紫斑牡丹中丹皮酚和芍药苷的提取。Objective To optimize the extraction process of paeonol and paeoniflorin in Paeonia rockii.Methods Based on the single-factor experiment,the Box-Behnken response surface methodology was used to optimize the optimal extraction process with the material-to-liquid ratio,extraction time,and ethanol volume fraction as influencing factors,and with the total weight comprehensive score of the extraction rates of paeonol and paeoniflorin as the evaluation indexes,and the validation test was conducted.Results The optimal extraction process was 28 times of liquid-solid ratio,150 min of extraction time,and 52%of ethanol volume fraction.Under the process conditions,the extraction rate of paeonol was 0.49%(RSD=1.33%,n=3),and the extraction rate of paeoniflorin was 5.38%(RSD=1.07%,n=3).Conclusion The extraction process was stable and feasible,which can be used for the extraction of paeonol and paeoniflorin from Paeonia rockii.

关 键 词：紫斑牡丹 丹皮酚 芍药苷 Box-Behnken响应面法 提取工艺 

分 类 号：R932[医药卫生—生药学] R284.2[医药卫生—药学] TQ460.6[化学工程—制药化工]