基于无透镜衍射成像的猪精子三维轨迹提取方法研究  

Study on Porcine Sperm 3D Trajectory Extraction Mehod Based on Lensless Diffraction Imaging

作　　者：李国晓 姜玥彤 李晨曦 武君伟 Li Guoxiao;Jiang Yuetong;Li Chenxi;Wu Junwei(School of Electrical and Information Engineering,Jiangsu Vocational College of Agriculture and Forestry,Zhenjiang,China;Lianyungang Lihua Animal Husbandry Co.,Ltd.,Lianyungang,China)

机构地区：[1]江苏农林职业技术学院信息工程学院,江苏镇江 [2]连云港立华牧业有限公司,江苏连云港

出　　处：《科学技术创新》2024年第3期22-25,共4页Scientific and Technological Innovation

摘　　要：精子活力检测是畜牧养殖领域生产过程中的一个重要环节。现有的计算机辅助精子分析(CASA)方法,利用显微镜拍摄精子运动视频,其评估的依据是由显微镜成像所得到的精子平面运动轨迹。事实上精子个体的运动是在三维空间中进行的。本研究提出了一种基于无透镜衍射成像的猪精子三维轨迹提取方法,通过对所拍摄的精液无透镜成像进行重建和图像处理,获得精子头部的平面定位,并针对重建过程中物像距离参数的准确性设计了评价函数,通过自聚焦算法锁定目标颗粒的物像距离,进而获得精子个体的三维运动轨迹。结果表明,该方法对精子个体的平面定位精度达到1.4μm,高度定位精度达到±5μm,能够获得较高精度的三维运动轨迹,有望为精子质量评估和生殖医学研究提供更加科学的数据支持。Sperm motility detection is an important step in the production process of animal husbandry.The existing method of computer-assisted sperm analysis(CASA),which uses a microscope to capture video of sperm movement,is evaluated based on the planar motion trajecars of sperm obtained by the microscope imaging.In fact,the movement of individual sperm is carried out in three dimensions.In this study,a method of 3D trajectory extraction of pig sperm based on lensless diffraction imaging was proposed.The plane location of sperm head was obtained through reconstruction and image processing of the lensless semen imaging captured.An evaluation function was designed for the accuracy of object image distance parameters in the reconstruction process,and the object image distance of the target particle was locked by self-focusing algorithm.Then the three-dimensional motion trajectories of individual sperm are obtained.The results showed that the proposed method could achieve a planar positioning accuracy of 1.4μm and a height positioning accuracy of±5μm for individual sperm,and could obtain a relatively high precision three-dimensional motion trajectory,which is expected to provide more scientific data support for sperm quality assessment and reproductive medicine research.

关 键 词：猪精子 三维轨迹 无透镜成像 衍射重建 聚焦度评价 

分 类 号：TP391.41[自动化与计算机技术—计算机应用技术] R446.1[自动化与计算机技术—计算机科学与技术]