白菜型油菜IPT基因家族鉴定及表达分析  

Identification and Characterization of IPT Gene Family in Brassica rapa L.

作　　者：刘博[1,2] 王旺田[1,2] 马骊[2] 武军艳[2] 蒲媛媛[2] 刘丽君[2] 方彦 孙万仓[2] 张岩[1,2] 刘睿敏 曾秀存[3] LIU Bo;WANG Wangtian;MA Li;WU Junyan;PU Yuanyuan;LIU Lijun;FANG Yan;SUN Wancang;ZHANG Yan;LIU Ruimin;ZENG Xiucun(College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;State Key Laboratory of Aridland Crop Science,Gansu Agricultural University,Lanzhou 730070,China;Hexi University,Gansu Zhangye 734000,China)

机构地区：[1]甘肃农业大学生命科学技术学院,兰州730070 [2]甘肃农业大学省部共建干旱生境作物学国家重点实验室,兰州730070 [3]河西学院,甘肃张掖734000

出　　处：《中国农业科技导报》2024年第2期56-66,共11页Journal of Agricultural Science and Technology

基　　金：甘肃农业大学干旱生境作物学重点实验室开放课题项目(GSCS-2020-06);甘肃省科技计划项目(21JR7RA835);国家现代农业产业技术体系建设项目(CARS-12);国家自然科学基金项目(31860388,31960435);甘肃省教育厅2021年度青年博士基金项目(2021QB-113);甘肃省高等学校创新基金项目(2022A-054)。

摘　　要：异戊烯基转移酶(isopentenyltransferase,IPT)是细胞分裂素生物合成的第一限速酶,为分析IPT基因家族在白菜型油菜生长中的功能,利用生物信息学方法从其基因组中鉴定出13个BrIPT基因,它们不均匀地分布在7条染色体上。BrIPTs可分为4个亚族,各家族成员均含有8~10个保守基序和1~2个UTR区。BrIPT基因的启动子区域包含众多响应元件。BrIPT基因家族成员受环境因子、生物激素及防御与应激调控。qRTPCR结果显示,tRNA-IPT基因BrIPT4、BrIPT6、BrIPT9在白菜型油菜体内各个部位均有表达。相比于苗期,成熟期白菜型油菜IPT基因的表达量更高,为后续深入研究IPT基因家族成员的生理生化功能提供了理论依据。Isopentenyltransferase(IPT)is the first limiting enzyme for cytokinin biosynthesis,so to analyze the function of the IPT gene family in Brassica rapa L.,a total of 13 BrIPT genes were identified from the genome of Brassica rapa L.by bioinformatics,which were heterogeneously distributed on 7 chromosomes.These BrIPT genes were divided into 4 substrates and each BrIPT gene included 8~10 conserved motifs and 1~2 UTR regions.The promoter regions of BrIPT genes contained numerous responsive elements.The BrIPT genes were regulated by environmental factors,biohormones,defense and stress.The qRT-PCR results showed that the tRNA-IPT genes BrIPT4,BrIPT6 and BrIPT9 were expressed in various parts of Brassica rapa L..The expressions of IPT genes in Brassica rapa L.were higher at the mature stage than those at the seedling stage.The above results provided some basis for subsequent in-depth studies on the physiological and biochemical functions of IPT gene family members.

关 键 词：白菜型油菜 IPT基因家族 细胞分裂素 生物信息学分析 

分 类 号：S565.4[农业科学—作物学]