具几丁质降解活性雪灵芝内生菌的筛选与鉴定  

Screening and Identification of Endophytes from Arenaria pulvinata Edgew. with Chitin-Degrading Activity

作　　者：廖会君 王瑞红[1,2,3] 崔月[1,2,3] 王贵双 宋福行 张新军 Liao Huijun;Wang Ruihong;Cui Yue;Wang Guishuang;Song Fuhang;Zhang Xinjun(Research Institute of Xizang Plateau Ecology,Xizang Agricultural&Animal Husbandry University,Nyingchi Xizang 860000,China;Key Laboratory of Forest Ecology of Xizang Plateau,Ministry of Education,Nyingchi Xizang 860000,China;National Forest Ecosystem Observation&Research Station of Nyingchi Xizang,Nyingchi Xizang 860000,China;Luntai Bureau of Education,Science and Technology,Bazhou Xinjiang 841600,China;Aquatic Science Institute,Xizang Academy of Agriculture and Animal Husbandry Science,Lhasa 850030,China;School of Light Industry,Beijing Technology and Business University,Beijing 100048,China)

机构地区：[1]西藏农牧学院高原生态研究所,西藏林芝860000 [2]西藏高原森林生态教育部重点实验室,西藏林芝860000 [3]西藏林芝高山森林生态系统国家野外科学观测研究站,西藏林芝860000 [4]新疆轮台县教育和科学技术局,新疆巴州841600 [5]西藏自治区农牧科学院水产科学研究所,拉萨8850030 [6]北京工商大学轻工科学技术学院,北京100048

出　　处：《蚕业科学》2023年第6期575-580,共6页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金项目(31960013);西藏自治区科技计划项目(XZ202101YD0013C);西藏自治区科技创新基地自主研究项目(XZ2022JR0007G);中国农业大学-西藏农牧学院科研联合基金项目(2022TC126);家蚕基因组生物学国家重点实验室开放课题研究基金项目;西藏农牧学院研究生教育创新计划项目(YJS2021-22)。

摘　　要：昆虫是危害农林作物的主要害虫,利用微生物防治有害昆虫是减少农林作物损失的有效途径之一。植物内生菌是生防菌的重要来源。以家蚕蛹外皮几丁质为筛选培养基主要成分,从西藏垫状雪灵芝(Arenaria pulvinata Edgew.)内生菌中筛选产高活性几丁质酶的菌株。透明圈法与DNS法酶活性测定相结合,筛选出一株降解几丁质能力较强、酶活性较高的菌株ZLB-158,根据形态学特征与16S rRNA基因序列鉴定,初步将该菌株归属为侧孢短芽孢杆菌(Brevibacillus laterosporus)。该菌株在蚕蛹胶体几丁质平皿上培养48 h形成的降解圈与菌落直径比为2.6,初始发酵条件下酶活力为0.404 U/mL。ZLB-158菌株产几丁质酶活性较高,在昆虫害虫防治方面具有潜在应用价值。Insects are the major pests that harm agroforestry crops. One of the effective ways to reduce the economic loss is microbial control method. Plant endophytes are important source of biocontrol agents. In this study, a bacterial strain ZLB-158 with strong chitin degradation ability and high chitinase activity was screened from the endophytes of Arenaria pulvinata Edgew. of Xizang with chitin from the outer skin of silkworm pupa being the main component of the screening medium, and by combining the transparent circle method and DNS method for enzyme activity determination. Based on morphological characteristics and 16S rRNA gene sequencing, the strain was preliminarily classified as Brevibacillus laterosporus. The ratio of the transparent circle to colony diameter was 2.6 after the colonies were cultured on chitin agar plates for 48 h. Under the initial fermentation conditions, the chitinase activity was 0.404 U/mL. ZLB-158 exhibited high chitinase activity and has potential application value in the control of insect pests.

关 键 词：蚕蛹 胶体几丁质 垫状雪灵芝 内生菌 几丁质降解菌 

分 类 号：S888.71[农业科学—特种经济动物饲养]