雾水葛总黄酮的提取工艺优化及其对线虫应激能力的影响  

Optimization of extraction technology of total flavonoids from Pouzolzia zeylanica(L.)Benn and its effect on stress ability of nematode

作　　者：吴冬凡[1] 李美其 王艳[1] WU Dongfan;LI Meiqi;WANG Yan(Guangdong Pharmaceutical University,Guangdong Cosmetics Engineering&Technology Research Center,Guangzhou 510006,China)

机构地区：[1]广东药科大学广东省化妆品工程技术研究中心,广东广州510006

出　　处：《广东药科大学学报》2024年第1期43-49,共7页Journal of Guangdong Pharmaceutical University

基　　金：广东省基础与应用基础研究基金项目(2019A1515011155);广东省中医药管理局项目(20221223)。

摘　　要：目的 正交法优化雾水葛总黄酮的提取工艺,探究其对秀丽隐杆线虫抗应激能力的影响。方法采用NaNO_(2)-Al(NO_(3))_(3)-NaOH显色法测定雾水葛中总黄酮的质量分数,以雾水葛总黄酮提取率为指标,通过单因素考察及正交试验考察时间、温度、料液比、乙醇体积分数对总黄酮提取率的影响,优选出最佳提取工艺。设立给药组和空白组,通过观察线虫头摆动次数、H_(2)O_(2)氧化应激、抗热应激试验探究总黄酮对秀丽隐杆线虫抗应激能力的影响。结果最佳提取工艺:提取时间为40 min,提取温度为70℃,乙醇体积分数为60%,料液比为1∶40 (g∶mL),总黄酮提取率实测值为1.31%。给药组线虫头摆动次数明显多于空白组(P<0.01)。雾水葛总黄酮质量浓度为0.300 mg/mL时,给药组在氧化应激和热应激实验中平均寿命比空白组分别延长32.28%、42.96%。结论超声法操作简单、便捷、提取率高,提取所得总黄酮能增强秀丽隐杆线虫应激能力。Objective To optimize the extraction process of total flavonoids from Pouzolzia zeylanica(L.)Benn by orthogonal design and explore its effect on the anti-stress ability of Caenorhabditis elegans.Methods The content of total flavonoids in Pouzolzia zeylanica(L.)Benn was determined by NaNO_(2)-Al(NO_(3))_(3)-NaOH color developing method.Using the extraction rate of total flavonoids from Pouzolzia zeylanica(L.)Benn as the indicator,The effects of extraction time,temperature,ratio of solid to liquid and concentration of ethanol on total flavonoids extraction were investigated by single factor and orthogonal design,and the optimal extraction process was selected.By setting up a medication group and a blank group,the effects of total flavonoids on the anti-stress ability of C.elegans were studied by observing the head swing times,H2O2 oxidative stress and anti-heat stress experiments.Results The optimum extraction technology of total flavonoids was as follows:the extraction time was 40 min,the extraction temperature was 70℃,the concentration of ethanol was 60%,and the ratio of solid to liquid was 1∶40(g∶mL).The actual extraction rate of total flavonoids was 1.31%.The number of head swing in the treated group was significantly higher than that in the control group(P<0.01).The mass concentration of total flavonoids in Pouzolzia zeylanica(L.)Benn was 0.300 mg/mL,the average lifespan of the experimental group was prolonged by 32.28%in oxidative stress and 42.96%in heat stress.Conclusion The ultrasonic method is simple,convenient and has high extraction rate.The extracted total flavonoids can enhance the stress ability of C.elegans.

关 键 词：雾水葛总黄酮 正交法优化 秀丽隐杆线虫 氧化应激 热应激 

分 类 号：R284.1[医药卫生—中药学]