HPLC法测定骨伤跌打康复凝胶贴膏剂中7种成分含量  

Determination of seven components in Gushang Dieda Kangfu Cataplasm by HPLC

作　　者：吴谋 莫楚铭 蓬展鹏 刘淑芳 陈继英 WU Mou;MO Chuming;PENG Zhanpeng;LIU Shufang;CHEN Jiying(Dongguan Hospital of Traditional Chinese Medicine,Dongguan 523000,China;Dongguan Key Laboratory of Traditional Chinese Medicine Preparations,Dongguan 523000,China)

机构地区：[1]东莞市中医院,广东东莞523000 [2]东莞市中药制剂重点实验室,广东东莞523000

出　　处：《广东药科大学学报》2024年第1期56-61,共6页Journal of Guangdong Pharmaceutical University

基　　金：东莞市社会发展科技项目重点项目(20211800904392)。

摘　　要：目的 建立测定骨伤跌打康复凝胶贴膏剂中7种主要成分质量分数的HPLC法。方法 色谱柱为Agilent ZORBA×SB-C18S tableBond Analytical柱(4.6 mm×250 mm, 5μm);测定波长为290 nm,流动相为乙腈-0.1%甲酸水梯度洗脱;流速为1.0 mL/min;柱温为35℃;进样量为10μL。结果 骨伤跌打康复凝胶贴膏剂中的原儿茶酸、咖啡酸、阿魏酸、盐酸小檗碱、大黄酸、大黄素、大黄酚7种主要有效成分在浓度线性范围内与色谱峰面积的标准曲线线性关系良好;原儿茶酸、咖啡酸、阿魏酸、盐酸小檗碱、大黄酸、大黄素、大黄酚的平均回收率分别为93.79%、95.86%、96.12%、95.31%、96.58%、107.43%、97.12%,7种成分的平均回收率均在93.79%~107.43%范围内。结论 建立的方法适合作为骨伤跌打康复凝胶贴膏剂质量控制的方法。Objective To establish an HPLC method for the determination of the seven main components of Gushang Dieda Kangfu Cataplasm.Methods The chromatographic column was Agilent ZORBA×SB-C18 S tableBond Analytical column,(4.6 mm×250 mm,5µm),the detection wavelength was 290 nm,and the mobile phase was acetonitrile-0.1%formic acid water by gradient elution at a flow rate 1.0 mL/min.The column temperature was 35℃,the injection volume was 10µL.Results There were good linear relationships between the standard curves of the chromatographic peak area and the concentration linear range of protocatechuic acid,caffeic acid,ferulic acid,berberine hydrochloride,rhein,emodin and chrysophanol in the Gushang Dieda Kangfu Cataplasm.The average recoveries were 93.79%,95.86%,96.12%,95.31%,96.58%,107.43%,97.12%,and they were between 93.79%-107.43%.Conclusion This established method is suitable for the quality control of the Gushang Dieda Kangfu Cataplasm.

关 键 词：骨伤跌打康复凝胶贴膏剂 高效液相色谱法 原儿茶酸 咖啡酸 阿魏酸 盐酸小檗碱 大黄酸 大黄素 大黄酚 

分 类 号：R284.1[医药卫生—中药学]