葛根沙棘颗粒对急性酒精性肝损伤小鼠的护肝作用研究  

Study on the protective effect of Pueraria Seabuckthorn granules on acute alcohol-induced liver injury

作　　者：高慧艳[1] 袁鹏[1] 李江敏[1] 陈东方[1] 徐冰[1] 张聪恪[1] GAO Huiyan;YUAN Peng;LI Jiangmin;CHEN Dongfang;XU Bing;ZHANG Congke(Henan Center for Disease Control and Prevention,Zhengzhou 450016,China)

机构地区：[1]河南省疾病预防控制中心,河南郑州450016

出　　处：《现代疾病预防控制》2024年第1期39-42,共4页MODERN DISEASE CONTROL AND PREVENTION

基　　金：中国疾病预防控制中心公共卫生领域卫生健康标准化前期研究项目(BZ2022-Q037);河南省重点研发与推广专项(科技攻关)项目(232102231040);河南省医学科技攻关计划联合共建项目(LHGJ20200134,LHGJ20220152)。

摘　　要：目的 研究葛根沙棘颗粒对急性酒精性肝损伤小鼠的保护作用。方法 将60只昆明小鼠随机分为空白对照组、模型对照组、剂量组(包括低、中、高3个剂量组,葛根沙棘颗粒给予剂量分别为0.25、0.50、1.50 g/kg),每组12只。除空白对照组外,其他组均建立急性酒精性肝损伤模型,剂量组给予葛根沙棘颗粒连续灌胃30 d。实验期结束测定小鼠肝组织中丙二醛(MDA)、谷胱甘肽(GSH)、甘油三酯(TG)的含量,并进行肝脏病理组织学检查。结果 模型组小鼠肝脏MDA、GSH、TG的含量及病理学评分与空白对照组相比差异有统计学意义(P均<0.05),表明急性酒精性肝损伤模型造模成功。葛根沙棘颗粒高剂量组小鼠肝组织匀浆MDA含量为(2.44±0.39)nmol/mg、GSH为(0.25±0.07)mg/g、TG为(0.022±0.007)mmol/g、病理评分为(2.75±0.97)分,模型对照组MDA为(3.27±0.68)nmol/mg、GSH为(0.17±0.09)mg/g、TG为(0.029±0.005)mmol/g、病理评分为(3.83±0.39)分;与模型对照组相比,葛根沙棘颗粒高剂量组能够降低酒精性肝损伤小鼠肝组织中MDA、TG含量,升高GSH含量,病理评分降低(P均<0.05)。结论 葛根沙棘颗粒对急性酒精性肝损伤小鼠具有保护作用。Objective To study the protective effects of Pueraria Seabuckthorn granules on acute alcohol-induced liver injury model in mice.Methods Sixty kunming mice were randomly divided into blank control group, model control group and dose groups(including three groups, low, medium and high dose groups, and the doses of Pueraria Seabuckthorn granules for the three groups were 0.25, 0.50 and 1.50 g/kg, respectively.). Each group had 12 mice. Except from the blank control group, acute alcoholic liver injury model was established in other groups. The mice in the dose groups were given oral gavage with corresponding doses of Pueraria Seabuckthorn granules for 30 days. The contents of malondialdehyde(MDA), glutathione(GSH)and triglycerides(TG) in liver issue of the mice at the end of the experiment were measured, and the pathologic examination of hepatic tissue was conducted. Results The contents of MDA, GSH and TG in liver and pathological scores in the model group were significantly different from those in the blank control group(all P<0.05), indicating that the model of acute alcoholic liver injury was successfully constructed. In the high dose group of Pueraria Seabuckthorn granules, MDA, GSH and TG contents in liver homogenate of mice were(2.44±0.39) nmol/mg,(0.25±0.07) mg/g and(0.022±0.007) mmol/g, respectively, and pathological score was(2.75±0.97). In the model control group, MDA, GSH and TG contents were(3.27±0.68) nmol/mg,(0.17±0.09) mg/g and(0.029 ±0.005) mmol/g, respectively, and pathological score was(3.83 ±0.39). Compared with the model control group, the contents of MDA and TG in liver tissue of alcohol-induced liver injury mice were decreased and the contents of GSH were increased, and hepatocyte steatosis score decreased in the high-dose group of Pueraria Seabuckthorn granules(all P<0.05).Conclusions Pueraria Seabuckthorn granules has the protective effects on acute alcohol-induced liver injury in mice.

关 键 词：葛根沙棘 小鼠 急性酒精性肝损伤 护肝 

分 类 号：R285[医药卫生—中药学]