粉防己碱减轻IL-1β诱导的体外人关节软骨细胞损伤  

Tetrandrine attenuatesIL-1β-induced injury of human articular chondrocytes in vitro

作　　者：欧阳云朗 张宁[1] 陈丽萍 贾丙申[1] 焦拓 OUYANG Yunlang;ZHANG Ning;CHEN Liping;JIA Bingshen;JIAO Tuo(Department of Joint Surgery,the First Affiliated Hospital of Hainan Medical College,Haikou 570000,China)

机构地区：[1]海南医学院第一附属医院关节外科,海南海口570000

出　　处：《基础医学与临床》2024年第2期174-179,共6页Basic and Clinical Medicine

基　　金：海南省卫生健康行业科研项目(20A200025)。

摘　　要：目的 粉防己碱(Tet)对白细胞介素1β(IL-1β)诱导原代人关节软骨细胞损伤的影响。方法 将人关节软骨细胞分为对照组、IL-1β组、低氧诱导因子-1α(HIF-1α)抑制剂[二甲氧基雌二醇(2-ME2)]组、Tet低、中、高浓度组,每组设置6个重复。MTT法检测细胞增殖;流式细胞仪检测细胞凋亡;ELISA检测细胞中炎性相关因子肿瘤坏死因子-α(TNF-α)、基质金属蛋白酶3(MMP-3)、诱导性一氧化氮合酶(iNOS)、环氧合酶-2(COX-2)的水平以及抗氧化因子超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPx)的活性;Western blot检测细胞中HIF-1α、VEGF蛋白的表达。结果 与对照组相比,IL-1β组细胞的凋亡率、TNF-α、MMP-3、iNOS、COX-2的水平以及HIF-1α、VEGF蛋白表达均升高,细胞存活率、SOD和GPx活性均降低,差异有统计学意义(P<0.05);与IL-1β组相比,2-ME2组和Tet低、中、高浓度组细胞的凋亡率、TNF-α、MMP-3、iNOS、COX-2的水平以及HIF-1α、VEGF蛋白表达均降低,细胞存活率、SOD和GPx活性均升高,差异有统计学意义(P<0.05)。结论 粉防己碱减轻IL-1β诱导的人关节软骨细胞损伤。Objective To investigate the effect of tetrandrine(Tet)on injury of primary human articular chondrocytes induced by interleukin-1β(IL-1β).Methods Human articular chondrocytes were divided into control group,IL-1βgroup,hypoxia inducible factor(HIF-1α)inhibitor group[2-methoxyestradiol(2-ME2)group],Tet groups containing low,medium and high concentrations.Each group has six replicated samples.MTT assay was used to detect the viability of cells;cell apoptosis was detected by flow cytometry;the level of inflammatory related factors like tumor necrosis factor-α(TNF-α),matrix metalloproteinase 3(MMP-3),inducible nitric oxide synthase(iNOS),cyclooxygenase-2(COX-2)and the activity of antioxidant factors like super oxide dismutase(SOD)and glutathione peroxides(GPx)in cells were detected by ELISA;Western blot was used to detect the expression of HIF-1αand VEGF in cells.Results Compared with the control group,the apoptosis rate,level of TNF-α,MMP-3,iNOS,COX-2 and the protein expression of HIF-1αand VEGF in IL-1βgroup all increased,the cell survival rate and the activity of SOD and GPx significantly decreased(P<0.05);compared with IL-1βgroup,the apoptosis rate,the level of TNF-α,MMP-3,iNOS,COX-2 and the protein expression of HIF-1αand VEGF in 2-ME2 group and Tet groups with low,medium,and high concentration significanthy decreased(P<0.05).The cell survival rate and the activity of SOD and GPx significantly increased(P<0.05).Conclusions Tetrandrine attenuates IL-1β-induced injury of human articular chondrocytes.

关 键 词：粉防己碱 骨关节炎 白细胞介素1Β 关节软骨细胞 损伤 

分 类 号：R684.3[医药卫生—骨科学]