小麦条锈菌新菌系CYR34中CDK5基因的克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of CDK5 Gene from a New Wheat Stripe Rust Strain CYR34

作　　者：蒋应磊 罗超 陈诗雯 邵欣 邹一萍 陶飞 薛应钰 JIANG Yinglei;LUO Chao;CHEN Shiwen;SHAO Xin;ZOU Yiping;TAO Fei;XUE Yingyu(College of Plant Protection,Gansu Agricultural University,Lanzhou 730070 China;College of Agriculture,Guangxi University,Nanning 530004,China;College of Life Science,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]甘肃农业大学植物保护学院,兰州730070 [2]广西大学农学院,南宁530004 [3]南京农业大学生命科学学院,南京210095

出　　处：《西北农业学报》2024年第1期133-140,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然基金地区科学基金(32060595);甘肃省青年基金(20JR10RA549);甘肃农业大学人才专项(GAU-KYQD-2018-37)。

摘　　要：为探究细胞周期蛋白依赖性激酶(Cyclin-dependentkinase5,CDK5)在小麦条锈菌(Pucciniastri-iformisf.sp.tritici)新菌系CYR34夏孢子萌发过程的毒性作用,以天水地区小麦条锈菌新菌系CYR34夏孢子标样为试材,通过RACE克隆CDK5基因并对其进行生物信息学分析,获得长度为706bp,编码190个氨基酸的cDNA序列。蛋白质结构预测显示,其二级结构主要以α-螺旋为主,且具有典型的STKC激酶结构域。同源性分析显示,CDK5与小麦杆锈菌(Pucciniagraminisf.sp.tritici)中的CDK5亲缘关系较近。蛋白质互作数据库预测分析发现,CDK5可以与磷酸核酮糖3-差异构酶、荚膜生物合成蛋白、鸟苷酸激酶、丝氨酸/苏氨酸特异性蛋白激酶、16SrRNA蛋白以及肌苷-5'-单磷酸脱氢酶6个蛋白互作关系;基因时序表达发现在孢子萌发0~6h时,基因的表达量持续下调,6h后表现为上调,萌发10h后为对照的1.2倍,萌发14h后基因的表达量达趋于平台期,为对照的1.36倍。综上所述,推测细胞周期蛋白依赖性激酶CDK5在小麦条锈菌(CYR34)夏孢子萌发过程中参与了适应环境的信号调控。In this study,we explored the toxic effect of cyclin-dependent kinase 5(CDK5)on the ger-mination of urediospores from the newly idencified CYR34 rust strain in Tianshui.The CDK5 gene was cloned ussing RACE(Rapid Amplifiction of cDNA Ends)and analyzed through bioinformatics tools.We successfully obtained a 706 bp cDNA sequence capable of encoding 190 amino acid.The structural prediction of protein showed a predominantαspiral secondary structure,with a characteris-tic STKC kinase domain.The homolology analysis demonstrated that CDK5 shared a closer relation-ship with the CDK5 found in Puccinia graminis f.sp.tritici.Moreover,based on the prediction and the analysis of CDK5 protein interaction network using PPIs database,CDK5 was shown to interact with six proteins,including ribose phosphate-3 isomerase,capsule biosynthetic protein,guanylate ki-nase,serine/threonine-specific protein kinase,16S rRNA protein and inosine-5'-monophosphate dehy-drogenase.Furthermore,the gene expression analysis indicated that CDK5 expression was initially down-regulated during 0-6 hours post germination(hpg)of urediospores,followed by an up-regula-ted after 6 hpg,reaching a peak at 14 hpg,where was 1.36 times higher than the control.This suggests that CDK5 may paly a role in the regulating environmental signals during the germination of CYR34 urediospores.

关 键 词：小麦条锈菌 CDK5 基因克隆 生物信息学分析 

分 类 号：S435.121.42[农业科学—农业昆虫与害虫防治]