苹果GRF基因家族在非生物胁迫下的表达分析  

Expression Analysis of GRF Gene Family under Abiotic Stress in Apple

作　　者：高明刚 李明 段高飞 王滕飞 冯晓健 许瑞瑞[1] GAO Minggang;LI Ming;DUAN Gaofei;WANG Tengfei;FENG Xiaojian;XU Ruirui(Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong Province,College of Biology and Oceanography,Weifang University,Weifang Shandong 261061,China)

机构地区：[1]潍坊学院生物与海洋学院,山东省高等学校生物化学与分子生物学重点实验室,山东潍坊261061

出　　处：《西北农业学报》2024年第1期141-147,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：作物生物学国家重点实验室开放课题(2021KF06);山东省自然科学基金(ZR2022MC064)。

摘　　要：GRF(Growth Regulating Factor)基因家族是一类植物特有的转录因子,它在调控植物的生长发育、渗透胁迫等方面发挥重要作用,主要通过调控细胞增殖过程促进植物组织器官的生长,提高植物对环境胁迫的适应性,因此,研究GRF基因家族在逆境条件下的表达调控具有重要意义。利用生物信息学分析和实时荧光定量PCR技术研究苹果中12个GRF基因在干旱、盐害和温度胁迫条件下的表达情况。结果表明:苹果MdGRFs基因参与响应非生物胁迫,干旱、盐害、高温和低温条件下MdGRFs表达量多数有明显变化。分别用干旱、盐害处理苹果幼苗后有相同的4个MdGRFs基因呈上调表达趋势;分别用干旱、低温处理后也有4个基因有相似的诱导表达;MdGRF05和MdGRF07受到干旱、盐害和低温的诱导表达;盐胁迫处理后,12个MdGRFs基因中有8个明显上调,4个明显下调;高温条件下,MdGRF04、MdGRF05、MdGRF07、MdGRF09和MdGRF11基因表达上调略明显,MdGRF02、MdGRF03和MdGRF10表达均下调,其中高温12 h后,几乎检测不到MdGRF10的表达。Growth Regulating Factor(GRF)gene family is a kind of plant-specific transcription fac-tors,which play an important role in regulating the growth and development of plants and osmotic stress,mainly the role in promoting the growth of plant tissues and organs by regulating the cell pro-liferation process,and improving the adaptability to environmental stress in plants.Therefore,it is of great significance to study the expression regulation of GRF gene family under abiotic stress in plant.In this study,bioinformatics analysis and quantitative real-time PCR were used to detect the expres-sion of 12 MdGRFs genes under drought,salt and temperature stress in apple.The results showed that MdGRFs were involved in response to abiotic stress,and the expression level of MdGRFs changed sig-nificantly under drought,salt injury,high temperature and low temperature.MdGRF05,MdGRF06,MdGRF07,MdGRF08 and MdGRF11 showed similar regulation pattern,and the regulation of MdGRF09 and MdGRF12 was different under drought and cold stress.After salt stress,eight MdGRFs were significantly up-regulated and four MdGRFs were significantly down-regulated.Under high temperature stress,the expression of MdGRF04,MdGRF05,MdGRF07,MdGRF09 and MdGRF11 genes were slightly up-regulated,and the expression of MdGRF02,MdGRF03 and MdGRF10 were down-regulated.The expression of MdGRF10 could hardly be detected after 12 h of high temperature.

关 键 词：苹果 GRF基因家族 非生物胁迫 表达分析 

分 类 号：S661.1[农业科学—果树学]