基于转录组测序芒果抗细菌性角斑病SNP/In Del分析  

SNP/In Del Analysis of Mango Bacterial Black Spot Disease Based on Transcriptome Sequencing

作　　者：周思思 王露露 胡芳丽 何红[1] 柳凤[2] 张国辉 普金安 张翠英 沐云松 ZHOU Sisi;WANG Lulu;HU Fangli;HE Hong;LIU Feng;ZHANG Guohui;PU Jin’an;ZHANG Cuiying;MU Yunsong(Guangdong Ocean University Binhai Agricultural College,Zhanjiang Guangdong 524088,China;Key Laboratory for Post-harvest Physiology and Technology of Tropical Horticultural Products of Hainan Province,Key Laboratory of Tropical Fruit Biology,Ministry of Agriculture,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang Guangdong 524091,China;Huaping County Late Ripening Organic Mango Research Center,Huaping Yunnan 674800,China;Cash Crop Workstation,Xinping Yi and Dai Autonomous County,Xinping Yunnan 653404,China)

机构地区：[1]广东海洋大学滨海农业学院,广东湛江524088 [2]中国热带农业科学院南亚热带作物研究所,海南省热带园艺产品采后生理与保鲜重点实验室,农业部热带果树生物学重点实验室,广东湛江524091 [3]华坪县有机晚熟芒果研究中心,云南华坪674800 [4]新平彝族傣族自治县经济作物工作站,云南新平653404

出　　处：《西北农业学报》2024年第1期148-155,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：云南省科技厅科技计划(202104BI090012);海南省重点研发计划(ZDYF2021XDNY158)。

摘　　要：旨在挖掘与芒果抗细菌性角斑病紧密联系的SNP/InDel位点,以进一步揭示芒果抗细菌性角斑病的遗传多样性和分子机理。试验材料为细菌性角斑病高抗品种‘热农1号’和高感品种‘凯特’,分别对两个品种接病菌后0d、2d、6d的果皮进行转录组分析,以基因组‘红象牙’作为参考,鉴定并分析芒果中SNP/InDel位点的特征。结果表明,‘凯特’和‘热农1号’分别获得32.77Gb和36.83Gb的数据量,每个样本过滤后的Q30均高于90%。将reads比对到芒果参考基因组上,两个品种共检测到1213112个SNP位点,62888个InDel位点,主要分布在内含子区、外显子区、基因间区和基因上下游区域。SNP中转换位点和颠换位点分别为751006个(61.91%)和462106个(38.09%),其中转换型中A->G略多,而A->T在颠换型中占多数;In Del位点插入和缺失分别每个样本平均有18769和25015个。生物信息学分析表明,全部的SNP和InDel位点所在的差异基因,主要参与分子功能有代谢途径、应答刺激和生物学调控等过程。In order to explore the SNP/In Del loci closely associated with mango bacterial black spot disease,and further reveal the genetic diversity and molecular mechanism of mango bacterial black spot disease,the high resistance variety‘Renong No.1’and the high susceptible variety‘Keitt’were used as experimental materials,the pericarp transcriptome of the two varieties at 0 d,2 d,and 6 d was analyzed after pathogen exposure,and the genome‘Red Ivory’was used as a reference to identify and analyze the characteristics of SNP/In Del loci in mango.The results showed that‘Keitt’and‘Renong No.1’obtained 32.77 Gb and 36.83 Gb of data,respectively,and the filtered Q30 of each sample was higher than 90%.Reads were compared to the reference genome of mango.A total of 1213112 SNPS and 62888 In Del loci were detected in the two cultivars,which were mainly distribu-ted in the intronic region,exon region,intergene region and upstream and downstream region of the gene.There were 751006(61.91%)conversion sites and 462106(38.09%)inversion sites,respec-tively.A->G was slightly more in conversion type,while A->T was the majority in inversion type.There were 18769 insertions and 25015 deletions per sample,respectively.Bioinformatics analysis showed that all the SNPS and the differentially located genes in the In Del locus were mainly involved in the molecular functions which include metabolic pathways,response stimulation and biological reg-ulation.

关 键 词：芒果 细菌性角斑病 转录组测序 单核苷酸多态性 插入缺失标记 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治]