采后黄瓜在冷驯化处理过程中的转录组变化  

Transcriptomic Changes of Postharvest Cucumber during Cold Acclimation

作　　者：王斌[1,2] 杨盼迪 王玉昆 蒋园园 WANG Bin;YANG Pandi;WANG Yukun;JIANG Yuanyuan(Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region,Shaoguan University,Shaoguan Guangdong 512005,China;College of Biology and Agriculture,Shaoguan University,Shaoguan Guangdong 512005,China)

机构地区：[1]韶关学院广东省粤北食药资源利用与保护重点实验室,广东韶关512005 [2]韶关学院生物与农业学院,广东韶关512005

出　　处：《西北农业学报》2024年第2期256-270,共15页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：广东省基础与应用基础研究基金(2022A1515011913)。

摘　　要：采后黄瓜是冷敏性果菜类蔬菜,在低温贮藏时易发生冷害。前期研究结果表明,冷驯化处理通过诱导采后黄瓜耐冷性,减少冷害发生。为探究冷驯化处理诱导的转录组学变化,以采后黄瓜为试材,分析冷驯化处理期间的转录组变化。与贮藏前(0 h)相比,在冷驯化处理12 h和72 h时,分别鉴定到1 870和3 550个差异表达基因。基因表达验证结果表明,RT-qPCR和转录组结果高度一致,证明转录组测序数据的准确性和可靠性。GO富集分析结果显示,冷驯化处理诱导的差异表达基因主要富集在氧化还原过程、细胞膜组分和转录因子活性3个GO途径中,表明冷驯化处理通过调节细胞膜组分、细胞内氧化还原状态,增强冷藏黄瓜耐冷性。进一步分析发现,104个转录因子基因响应冷驯化低温,差异表达的转录因子主要是ERF、bZIP、WRKY和HSF家族,表明转录因子介导的转录调控在冷驯化诱导的耐冷性中发挥重要作用。研究结果为采后黄瓜诱导耐冷性提供了新见解,有助于加深对冷驯化诱导耐冷性分子机理的认识,为耐冷黄瓜培育提供了重要基因资源。Postharvest cucumber is a kind of cold-sensitive fruit vegetable,which is subjected to chill-ing injury during cold storage.The results of previous studies showed that cold acclimation treatment could induce the chilling tolerance of postharvest cucumber and reduce the occurrence of chilling inju-ry.In order to explore the transcriptomic changes induced by cold acclimation,transcriptomic changes during cold acclimation were analyzed.Compared with that before storage(0 h),cold acclimation sig-nificantly affected the differential expression of 1870 and 3550 genes at 12 h and 72 h,respectively.The expression verification results of representative differentially expressed genes(DEGs)showed that the results obtained by qRT-PCR and RNA-seq were consistent in general,which conformed the accuracy and reliability of RNA-seq data.The results of GO enrichment analysis showed that the DEGs induced by cold acclimation were mainly enriched in three GO pathways:oxidation-reduction process,integral component of membrane and transcription factor activity,suggesting that cold accli-mation enhanced the chilling tolerance of cucumber fruit by regulating oxidation-reduction status and cell membrane component.Further analysis showed that 104 transcription factor genes were respon-ded to cold acclimation,and differentially expressed transcription factors were mainly ERF,bZIP,WRKY and HSF families,indicating that transcription regulation mediated by transcription factor played an important role in cold-acclimation-induced chilling tolerance in cucumber fruit.The results provide new insights into the induction of chilling tolerance in harvest cucumbers,and help to deepen the understanding of the molecular mechanisms of chilling tolerance induced by cold acclimation,and identify important gene resources for the cultivation of cold-tolerant cucumber.

关 键 词：采后黄瓜 耐冷性 冷驯化 转录组学分析 转录因子 

分 类 号：S642.2[农业科学—蔬菜学]