内生真菌对宿主布顿大麦耐碱性的渗透调节和膜系统影响  

Effects of Endophytic Fungi on Osmotic Regulation and Membrane System of Alkaline Tolerance in Host Hordeum bogdanii Barley

作　　者：任改香 陈水红[2] 王凯 特木尔布和 REN Gaixiang;CHEN Shuihong;WANG Kai;Temuerbuhe(College of Grassland and Resource Environment,Inner Mongolia Agricultural University,Hohhot 010019,China;College of Life Science and Technology,Tarim University,Alar Xinjiang 843300,China;College of Animal Science and Technology,Tarim University,Alar Xinjiang 843300,China)

机构地区：[1]内蒙古农业大学草原与资源环境学院,呼和浩特010019 [2]塔里木大学生命科学与技术学院,新疆阿拉尔843300 [3]塔里木大学动物科学与技术学院,新疆阿拉尔843300

出　　处：《西北农业学报》2024年第2期280-286,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(31660689,31802132);“兵团英才”第二期第三层人才项目。

摘　　要：为了研究带内生真菌(E+)和不带内生真菌(E-)布顿大麦(Hordeum bogdanii)的耐碱性,选择来源于新疆阿克苏温宿县的带内生真菌(E+)和不带内生真菌(E-)布顿大麦种子,先在培养皿中进行发芽,然后进行室内蛭石无土栽培,对其进行21 d的不同浓度混合碱胁迫处理,测定其渗透调节和膜系统变化。结果表明,在相同混合碱处理下,E-植株的相对电导率均显著高于E+植株(P<0.05),E+植株的细胞通透性弱于E-植株。在碱胁迫下E+植株的脯氨酸、可溶性糖和可溶性蛋白含量均显著高于E-植株(P<0.05)。内生真菌具有提高宿主渗透调节物质和降低膜损伤来提高布顿大麦抗碱的能力。This study aimed to investigate alkaline tolerance of Hordeum bogdanii barley,comparing plants with endophytic fungi(E+)and those without endophytic fungi(E-).Seeds of alkaline-tol-erant barley from Aksu Wensu County in Xinjiang were used as experimental materials.Initially,the seeds were germinated in petri dishes and then cultivated in vermiculite soilless conditions in a labora-tory setting.Over a 21-day period,the changes in osmotic regulation and membrane system under treatments of different mixed alkali concentration were observed.The results showed that E-plants exhibited significantly higher relative electrical conductivity compared to E+plants when subjected to the same mixed alkali treatment(P<0.05),indicating greater cell membrane damage in E-plants.Conversely,the E+plants exhibited stronger cell permeability and higher levels of proline,soluble sugar,and soluble protein under alkali stress compared to E-plants(P<0.05).In conclusion,En-dophytic fungi play a role in enhancing the host’s osmotic regulation substances and reducing mem-brane damage,thereby improving alkali resistance of Hordeum bogdanii barley.

关 键 词：内生真菌 布顿大麦 混合碱胁迫 渗透调剂 膜系统 

分 类 号：S543.9[农业科学—作物学]